FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483660

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483660
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2927127
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT173890.5940637355331696Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT293800.0134.5062
ACTGCAA299850.0132.228551
TGCAATT322750.0130.766163
ACCGCAA35400.0125.8172761
CGCAATT36700.0124.254033
CCGCAAT35850.0123.561122
GCAATTT419500.0112.015164
AATTTCC442750.0109.413786
CAATTTC435550.0109.239965
TTTCCAC451500.0106.820758
ATTTCCA455400.0106.0809257
TTCCACA458200.0105.49419
CGGCAAT8750.066.502142
ATTGCAA21300.059.7930341
ACGGCAA11400.050.4329531
ATGCAAT32500.047.6705252
GGCAATT12250.047.4966433
TCCGATC8300.042.9365355
CCGATCT11000.034.904586
GCAATTC17100.034.450694
Produced by FastQC (version 0.11.5)