FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483668

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483668
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12666123
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT2969812.3446874785599348Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT1173250.0131.032762
ACTGCAA1187300.0130.010181
TGCAATT1326100.0125.829463
CGCAATT163450.0124.397493
ACCGCAA157350.0122.590931
CCGCAAT159400.0121.178092
GCAATTT1732450.0108.915534
AATTTCC1822000.0106.63596
CAATTTC1793250.0106.211525
TTTCCAC1838100.0105.01138
ATTTCCA1885550.0102.711257
TTCCACA1889750.0102.2639169
CGGCAAT33600.071.588992
ACGGCAA46950.051.724791
ATGCAAT176350.045.9469682
ATTGCAA106100.041.7339741
GGCAATT62750.040.1850623
GTTCCAC87000.037.4200365
TTCCGAT36250.036.0836074
TCCGATC37400.035.1683855
Produced by FastQC (version 0.11.5)