FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483670

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483670
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12864803
Sequences flagged as poor quality0
Sequence length151
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT181227314.087063750606985Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT414320.3220570109002058Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT257150.19988646542041882Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT198180.1540482197823006Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT136940.10644546986067335Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC133290.10360827134313678RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGGAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC129860.10094208205131475RNA PCR Primer, Index 16 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT1227750.0131.110672
ACTGCAA1247300.0129.443881
TGCAATT1377650.0126.69213
CGCAATT180500.0120.528583
CCGCAAT171600.0120.2163542
ACCGCAA179500.0115.152911
GCAATTT1830700.0108.074064
AATTTCC1915650.0106.603726
CAATTTC1882050.0106.209835
TTTCCAC1924500.0105.1680458
ATTTCCA1970150.0103.250017
TTCCACA1980950.0102.2406549
CGGCAAT33650.074.930722
GACCGGA52750.071.6302262
AGACCGG53550.070.600271
AGATTGG115650.061.1687661
GATTGGA115400.061.140482
ACCGGAA70150.054.0702363
ACGGCAA49000.050.7449151
ATTGGAA138750.050.5370333
Produced by FastQC (version 0.11.5)