FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483674

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483674
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6338110
Sequences flagged as poor quality0
Sequence length151
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT5145658.118587402238207Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT115320.18194698419560404Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT72800.11486073924245556Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT646000.0133.757312
ACTGCAA659550.0131.445771
TGCAATT734600.0127.2404253
CGCAATT88950.0125.010573
CCGCAAT88000.0121.818732
ACCGCAA91800.0117.5537341
GCAATTT982500.0107.3960344
AATTTCC1020000.0106.4486166
CAATTTC1007000.0105.828745
TTTCCAC1030650.0104.3138968
ATTTCCA1048000.0103.224137
TTCCACA1065400.0101.081619
CGGCAAT17000.077.381012
ACGGCAA24850.053.551291
GACCGGA21150.052.575792
GGCAATT26700.050.9016233
ATTGCAA50350.047.0829161
AGACCGG24100.047.070931
ATGCAAT90750.046.8504682
GTTCCAC42200.045.80895
Produced by FastQC (version 0.11.5)