FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483676

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483676
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10607410
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT1916001.8062844747209734Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCAA1304550.0134.29981
CTGCAAT1301850.0134.061832
ACCGCAA174600.0130.963291
TGCAATT1460400.0129.035573
CCGCAAT177350.0128.865542
CGCAATT185750.0128.044953
GCAATTT1938650.0110.17634
AATTTCC2018750.0108.896566
CAATTTC1987050.0108.501975
TTTCCAC2036800.0107.088038
ATTTCCA2074700.0105.589497
TTCCACA2082050.0104.819829
CGGCAAT30500.083.866822
ACGGCAA39800.064.1205441
ATGCAAT166650.054.201732
GGCAATT53750.049.8876653
ATTGCAA85950.047.455891
GTTCCAC72500.042.196165
GTAATTT105000.038.270554
GCAAGTT77550.038.1580121
Produced by FastQC (version 0.11.5)