FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483688

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483688
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12794298
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT5457794.265798717522446Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT1362450.0132.998112
ACTGCAA1373200.0132.4341
ACCGCAA176900.0128.457411
CCGCAAT179050.0126.970062
TGCAATT1565550.0124.852543
CGCAATT193450.0122.816043
GCAATTT2010450.0109.888914
CAATTTC2073150.0107.697775
AATTTCC2117300.0107.5150456
TTTCCAC2106950.0107.072968
TTCCACA2157300.0104.59419
ATTTCCA2174600.0104.2820367
CGGCAAT34150.074.062342
ACGGCAA45750.055.3127371
TCCGATC41200.047.8050965
ATTGCAA115550.047.3243751
TTCCGAT44850.043.5905084
GGCAATT62250.042.498243
CCGATCT49150.042.1815956
ATGCAAT207450.040.815152
Produced by FastQC (version 0.11.5)