FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483690

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483690
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9696562
Sequences flagged as poor quality0
Sequence length151
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT3547513.658523505547636Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT1159850.0136.533252
ACTGCAA1172000.0135.576861
CGCAATT167250.0131.723753
TGCAATT1306950.0131.017093
CCGCAAT162700.0129.825732
ACCGCAA167300.0126.525571
GCAATTT1711400.0113.9613654
AATTTCC1783650.0112.72256
CAATTTC1752800.0112.384775
TTTCCAC1796800.0111.1148458
ATTTCCA1829150.0109.557897
TTCCACA1846400.0108.302719
CGGCAAT28150.089.310192
GGCAATT37750.071.217353
ACGGCAA40050.062.618041
ATTGCAA73850.060.8304251
ATGCAAT135400.058.762292
TATGCAA48550.046.1152271
GTTCCAC64500.044.3844455
CGTAATT8150.042.7941743
Produced by FastQC (version 0.11.5)