FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483694

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483694
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8190744
Sequences flagged as poor quality0
Sequence length151
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT133902716.34805092186986Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT304050.37121169945001337Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT184190.2248757866196282Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT139470.17027757185427844Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT114990.14039017700956102Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC104480.12755861982745403RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGGAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC101660.12411570914681255RNA PCR Primer, Index 16 (96% over 25bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAA97330.11882925409462193No Hit
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT87230.10649826194055144Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGCAAAA81990.10010079670418218No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT1222500.0137.612782
ACTGCAA1237150.0136.506421
TGCAATT1366350.0131.553673
CCGCAAT174750.0129.479722
CGCAATT182700.0128.816863
ACCGCAA185850.0122.4356841
GCAATTT1846400.0111.044774
AATTTCC1908750.0110.8667146
CAATTTC1874400.0110.4700245
TTTCCAC1919100.0109.354688
ATTTCCA1935850.0108.869097
TTCCACA1974300.0106.326579
CGGCAAT29500.095.0748442
GATTGGA72500.075.667482
AGATTGG72650.075.250341
ACGGCAA40600.068.938361
GGCAATT41650.068.910033
ATTGGAA81800.067.5088353
AGACCGG37450.067.166161
GACCGGA37600.066.2836762
Produced by FastQC (version 0.11.5)