FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005483708

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483708
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10298472
Sequences flagged as poor quality0
Sequence length151
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT124320312.071722873063111Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT283570.2753515278771453Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT173060.168044346772997Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT129480.12572738946127154Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAA124340.12073635778200884No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGCAAAA103720.10071396999477204No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)