FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483714

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483714
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6515096
Sequences flagged as poor quality0
Sequence length151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT333579451.200995349876656Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT1316202.0202311677372062Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT1176871.8063739966379622Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT947891.4549133274475157Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT859221.3188140282199987Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT731731.1231300352289513Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT719331.1040973149129345Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
TGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT421530.6470050479685948Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGGTCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT381520.585593827013447Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
GGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT376870.5784565568949406Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGTTCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT281500.4320734491095757Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC203160.3118296338227403RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGGAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC202930.3114766075588142RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC168300.2583231313859381Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
AGAGCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT113680.17448706818748333Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGACACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC111980.17187774362802943RNA PCR Primer, Index 16 (100% over 22bp)
AGATCGGAAGAGACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC98940.15186268936021818TruSeq Adapter, Index 16 (95% over 22bp)
AGATCGGAAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTC98500.15118733476835952Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGAGCAACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC89300.13706628421131475TruSeq Adapter, Index 16 (95% over 22bp)
AGATAGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT80980.12429594283798735Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
CTCTTCCGATCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTC80460.12349779650215438Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGAGCACAGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC78070.11982939315092211TruSeq Adapter, Index 16 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCAATT328750.0127.405033
CTGCAAT281600.0126.034882
GACCGGA176200.0125.1812742
AGATTGG160600.0123.3472061
GATTGGA166100.0122.917772
AGACCGG177450.0122.883971
TTGGAAG176250.0119.662394
ACCGGAA187800.0119.648313
ATTGGAA174650.0119.265423
CCGGAAG190450.0119.2001954
GCAATTT419250.0117.073214
ACTGCAA296550.0116.563561
CAATTTC470750.0115.979685
AATTTCC506450.0114.793676
TGATCGG63650.0112.903691
ATTTCCA516700.0112.289617
ACATCGG103900.0112.132321
CATCGGA105600.0111.481162
GGATCGG58750.0110.706071
TTTCCAC525150.0110.165318
Produced by FastQC (version 0.11.5)