FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483716

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483716
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8098513
Sequences flagged as poor quality0
Sequence length151
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT147374118.197674066831777Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT607260.7498413597656755Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT552380.6820758329337744Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT435930.5382840034954565Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT400460.49448583956091696Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT321670.39719637419857207Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT317290.39178797391570525Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
TGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT197520.24389662645475782Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGGTCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT179100.22115171019667437Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
GGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT172680.21322432896014368Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGTTCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT136350.1683642416823928Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC105090.12976456295124797RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGGAAAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC101650.12551686957840286RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC84950.10489580000674198Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT486250.0129.107642
TGCAATT556100.0128.150573
ACTGCAA496800.0122.809021
GCAATTT705600.0118.258344
CGCAATT67500.0117.272043
CAATTTC793900.0117.0643545
AATTTCC846100.0116.416166
ATTTCCA880650.0111.463327
TTTCCAC886000.0110.332088
GACCGGA98500.0109.686572
AGACCGG97800.0109.068071
CCGCAAT66350.0103.8909452
TTCCACA943200.0103.8639149
ACCGGAA110800.099.0114753
CGGCAAT34650.092.34862
ACATCGG57050.092.214991
AGATTGG107700.090.013971
ATTGGAA110700.089.4005663
TGATCGG35450.089.246131
GATTGGA110450.088.950412
Produced by FastQC (version 0.11.5)