FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483718

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483718
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10714042
Sequences flagged as poor quality0
Sequence length151
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT170611615.924111553790809Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT686780.6410092474903496Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT626200.5844666280008982Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT493510.4606198108986319Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT446790.41701348566675395Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT371150.3464145464428831Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT369560.3449305126860619Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
TGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT222420.20759672213343947Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGGTCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT201370.1879496085604294Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
GGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT197010.1838801826612216Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGTTCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT152870.14268191220456294Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC108690.10144630756534274RNA PCR Primer, Index 16 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT604700.0125.303122
TGCAATT698600.0124.521273
ACTGCAA622250.0118.6554641
GCAATTT871500.0116.213274
CAATTTC984050.0113.915815
AATTTCC1046650.0112.9875266
CGCAATT87500.0111.9289553
GACCGGA108350.0110.280732
TTTCCAC1081200.0108.4874348
AGACCGG109200.0108.2365651
ATTTCCA1093650.0107.676657
CCGCAAT83750.0103.172012
TTCCACA1145500.0102.7331549
ACCGGAA124800.098.9958043
ACCGCAA87700.095.8869251
ACATCGG64750.095.13571
CGGCAAT37850.090.2796862
TGATCGG39750.087.2501751
CATCGGA72700.085.921872
ACGGAAG104850.083.649724
Produced by FastQC (version 0.11.5)