FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483724

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483724
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7912521
Sequences flagged as poor quality0
Sequence length151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT276460734.939648185451894Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT1102221.393007361370668Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT991841.2535069417193332Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT791170.9998962404017632Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT728630.9208569557034982Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT611630.7729900495682729Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT610700.7718146972374544Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
TGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT354460.44797353460420514Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGGTCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT320800.4054333631468403Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
GGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT317440.4011869289193672Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGTTCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT239260.30238150394798324Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC181080.22885247318774887RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGGAAAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC176560.22314000809602907RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC142800.18047345466760847Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
AGATCGGAAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTC107510.13587325708203493Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGACACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC95370.12053048579586709RNA PCR Primer, Index 16 (100% over 22bp)
AGAGCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT93610.11830616310528591Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGAGACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC83570.10561741321128879TruSeq Adapter, Index 16 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT367850.0125.4917762
TGCAATT428600.0124.79843
GACCGGA152500.0123.61232
AGACCGG151750.0122.559781
ACTGCAA380000.0118.567621
ACCGGAA167750.0114.9224553
GCAATTT542650.0114.674484
CAATTTC611900.0113.2555545
AATTTCC654550.0111.931576
GATTGGA150150.0111.339562
AGATTGG148400.0110.656951
CCGGAAG176950.0110.171244
ACATCGG94850.0110.090931
CGCAATT53350.0108.5286253
ATTTCCA676750.0107.884667
CATCGGA97400.0107.348842
TTTCCAC679850.0107.051548
TGATCGG54650.0106.75641
ATTGGAA164400.0103.406143
TTGGAAG169450.0101.046574
Produced by FastQC (version 0.11.5)