FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483730

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483730
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11730477
Sequences flagged as poor quality0
Sequence length151
%GC50

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT10092948.604032044050722Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT412760.35186974920116204Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT374430.3191941811061903Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT296750.25297351505825383Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT275500.2348583096833999Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT224770.19161198645204283Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT220720.1881594414276589Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
TGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT134540.11469269322978086Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGGTCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT121450.10353372671887086Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
GGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT118920.10137695167894707Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCAATT663450.0126.175033
CTGCAAT573650.0125.214892
ACTGCAA592050.0117.8933641
CGCAATT76850.0116.971093
GCAATTT843450.0115.0538944
CAATTTC950650.0113.210455
AATTTCC1009000.0112.9093256
TTTCCAC1072550.0105.4064258
ATTTCCA1078400.0105.2446757
TTCCACA1133050.099.778199
CCGCAAT78100.099.5890352
ACCGCAA84800.089.078461
GACCGGA84650.087.2546542
AGACCGG84250.086.042571
ACATCGG45700.082.566711
CGGCAAT40400.080.277522
ACCGGAA99400.076.347783
TGATCGG33700.071.4868241
GGCAATT60500.067.3956763
CATCGGA59450.064.4391562
Produced by FastQC (version 0.11.5)