FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483732

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483732
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7789331
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT4947456.351572426438162Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT201860.25914934158016906Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT184630.23702934180098392Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT144360.18533042182955123Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT132970.17070785668242883Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT107300.13775252329115298Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT103980.13349028305511731Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT502850.0128.780152
TGCAATT578950.0128.45333
ACTGCAA518900.0121.799171
CGCAATT63000.0120.69333
GCAATTT728200.0118.481324
CAATTTC823700.0116.2367555
AATTTCC877450.0115.622316
TTTCCAC911350.0110.643288
ATTTCCA915950.0110.3646247
CCGCAAT61650.0105.334142
TTCCACA960100.0105.0932169
ACCGCAA61150.0103.7111
CGGCAAT33650.094.2270362
GACCGGA46250.085.499572
AGACCGG46100.084.682021
ACGGCAA40100.076.000211
GGCAATT52550.074.556153
ACCGGAA59250.068.574413
ACATCGG28300.066.9212341
TGATCGG17900.064.859981
Produced by FastQC (version 0.11.5)