FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483738

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483738
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8133904
Sequences flagged as poor quality0
Sequence length151
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT113381313.939345731151978Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT461170.5669725140596693Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT414410.5094847443490851Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT329990.4056969445422518Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT302620.3720476661637511Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT253270.31137569363985607Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT249670.30694977467154766Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
TGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT150710.18528617992049085Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGGTCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT136310.167582504047257Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
GGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT129930.15973879209786593Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGTTCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT100180.12316348951253912Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT445900.0125.8700562
TGCAATT515350.0124.89963
ACTGCAA456200.0119.8374251
CGCAATT63200.0115.510263
GCAATTT659900.0114.0306554
CAATTTC747950.0111.9446265
AATTTCC789600.0111.929486
GACCGGA78150.0109.295732
TTTCCAC822050.0106.725888
ATTTCCA828500.0106.174997
CCGCAAT60400.0104.409162
AGACCGG81200.0104.034341
TTCCACA881850.099.7927259
ACCGGAA90900.096.596463
ACCGCAA66150.093.2542951
CGGCAAT30550.090.7419052
ACATCGG47200.089.7946851
TGATCGG29700.086.502091
CATCGGA54050.079.35012
ACGGAAG75100.076.525514
Produced by FastQC (version 0.11.5)