FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483742

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483742
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8179616
Sequences flagged as poor quality0
Sequence length151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT224801527.48313612766174Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT925701.1317157186841045Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT828951.0134338824707663Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT662650.8101236048244806Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT601940.7359025166951603Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT497310.6079869764057383Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT483280.5908345819657059Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
TGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT299340.36595849976331407Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGGTCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT270450.33063899332193586Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
GGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT265960.32514973808061404Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGTTCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT202430.2474810553453854Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC145280.17761224976820428RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGGAAAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC144820.1770498761800065RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC116390.1422927433268261Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
AGATCGGAAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTC107860.1318643809195933Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT374750.0123.826942
TGCAATT436000.0123.0502853
GACCGGA132600.0119.898872
AGACCGG131550.0119.3186951
GCAATTT542350.0115.493754
ACTGCAA397400.0113.507871
CAATTTC617900.0113.01685
AATTTCC659400.0112.109896
ACCGGAA145100.0111.5634163
TTTCCAC678550.0108.132748
ACATCGG77450.0107.188311
ATTTCCA688300.0107.001197
CGCAATT55650.0106.7845463
TGATCGG47250.0106.125331
CCGGAAG158100.0103.625944
CATCGGA82350.0102.214052
TTCCACA721400.0101.930889
GATTGGA142100.099.321162
AGATTGG139750.098.4530941
CCGCAAT54400.096.039492
Produced by FastQC (version 0.11.5)