FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483746

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483746
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6959406
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT2423073.4817195605487017Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT96450.1385894140965479Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT86340.12406231221457693Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCAATT508150.0126.309043
CTGCAAT441250.0125.468552
ACTGCAA435650.0123.9932561
GCAATTT638450.0117.803324
CGCAATT63450.0116.8688353
AATTTCC759400.0115.378026
CAATTTC719900.0115.243215
ACCGCAA55050.0114.680971
CCGCAAT57150.0113.632322
TTTCCAC793350.0109.601988
ATTTCCA799200.0109.226037
TTCCACA829800.0104.787599
CGGCAAT28500.089.87212
ACGGCAA35350.069.7941361
GACCGGA27050.067.597142
GGCAATT48150.067.207543
AGACCGG26250.067.17481
ATGCAAT90100.059.9965822
ACATCGG16650.053.6068531
CCAATTT58350.051.1062664
Produced by FastQC (version 0.11.5)