FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483748

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483748
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4046328
Sequences flagged as poor quality0
Sequence length151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT50135512.39036973769798Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT207120.5118715042379165Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT185860.4593300394827113Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT147960.3656648694816634Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT135070.3338088261752384Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT110790.2738038043381555Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT110020.2719008444199284Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
TGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT67090.16580465053747498Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGGTCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT62990.155672006817045Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
GGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT60140.14862858374308757Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGTTCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT44520.11002568254476651Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT238600.0125.431172
TGCAATT275050.0124.238393
ACTGCAA239850.0122.126121
CGCAATT33250.0117.609853
GCAATTT354750.0113.4780964
CAATTTC400550.0111.836565
AATTTCC429300.0110.6181266
GACCGGA30350.0109.9641342
CCGCAAT31250.0109.8153462
TTTCCAC440900.0107.084818
ATTTCCA448200.0105.647977
AGACCGG31600.0104.245731
TTCCACA463600.0102.1228949
ACCGCAA32450.0101.5151141
CGGCAAT16300.092.137132
ACATCGG22200.090.86181
ACCGGAA39050.088.8082663
TGATCGG11950.085.006021
CATCGGA24850.082.625092
GGTCGGA15450.075.135192
Produced by FastQC (version 0.11.5)