FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483750

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483750
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2181135
Sequences flagged as poor quality0
Sequence length151
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT30996814.211316585172398Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT126710.5809360722742976Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT113270.5193167777326941Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT89750.41148301228488837Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT83390.38232388183216537Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT69170.3171284675180583Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT67420.3091051218746203Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
TGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT41270.18921341411696205Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGGTCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT37580.17229561673165578Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
GGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT36290.16638126480020723Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAA28390.13016159018125883No Hit
AGTTCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT28350.12997819942369454Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGCAAAA24380.11177666673543821No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT342150.0140.201222
ACTGCAA333200.0139.642471
TGCAATT407950.0135.299553
CCGCAAT39950.0132.0642
CGCAATT46450.0129.357483
ACCGCAA41500.0125.041661
CGGCAAT19600.0122.557192
GGCAATT23550.0120.484933
CAATTTC598800.0119.670315
GCAATTT539150.0119.242524
AATTTCC640100.0118.3270346
ATTTCCA650500.0116.151337
CCAATTT24700.0115.746094
TTTCCAC663350.0113.398638
AGACCGG20500.0108.687921
GTTCCAC28050.0108.384775
TTCCACA703950.0106.9305049
GACCGGA21350.0106.732972
ACGGCAA22400.0102.384881
TCCAATT24500.098.337443
Produced by FastQC (version 0.11.5)