FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483754

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483754
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2828975
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT34921612.344258963051988Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAA44280.1565231223322935No Hit
AGATCGGAAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTC40900.1445753320548962Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGCAAAA38780.13708145176256417No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAT32070.11336261366749441No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGCATTT29300.10357108139873983No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCAA568250.0138.404831
CTGCAAT564800.0138.315782
CCGCAAT78000.0132.204352
TGCAATT682350.0129.866443
ACCGCAA80950.0128.027241
CGCAATT88600.0126.558163
CGGCAAT11100.0108.6783452
GCAATTT976250.0103.0600744
ATTGCAA35550.0102.699531
CAATTTC979900.0102.591285
AATTTCC978150.0102.5622566
TTTCCAC975450.0101.999268
ATTTCCA986600.0101.324127
TTCCACA990350.0100.713539
ACGGCAA14500.082.759961
GGCAATT16000.078.120753
TCGCAAT6700.073.75512
ATGCAAT80800.068.80312
AACGATT6550.063.0935066
AATGCAA52500.061.0918961
Produced by FastQC (version 0.11.5)