FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483756

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483756
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10801691
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT5978785.535040763524896Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAA110930.10269688329355099No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT1449600.0133.851522
ACTGCAA1457700.0133.576321
CCGCAAT191700.0127.770852
TGCAATT1670650.0126.709693
ACCGCAA196800.0125.485791
CGCAATT210550.0122.5780943
GCAATTT2285800.0104.732884
CAATTTC2322100.0102.98385
AATTTCC2320150.0102.774596
TTTCCAC2312850.0102.308778
TTCCACA2338400.0101.360969
ATTTCCA2362400.0100.5833057
CGGCAAT31050.081.201312
ACGGCAA42300.058.4508061
TATGCAA82050.055.215551
ATGCAAT209550.053.571772
ATTGCAA101100.050.9253541
GGCAATT57050.044.9587483
TCCGATC44650.040.3527345
TTCCGAT48550.036.9646644
Produced by FastQC (version 0.11.5)