FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483767

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483767
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2734467
Sequences flagged as poor quality0
Sequence length6
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGG106090.38797323207776874No Hit
TAACCT96490.3528658418624178No Hit
GGGTGG80560.29460951622381987No Hit
GGGGGC75190.27497131982210793No Hit
GTGGAG58210.21287512337870598No Hit
GGGCAG53090.1941511819305188No Hit
GAAGAG52190.19085986409782968No Hit
GGATGG52100.19053073231456075No Hit
GAGGGA51980.19009188993686887No Hit
GTAGTG51960.1900187495405869No Hit
GCAGAG49960.18270470991238877No Hit
AGGTTA49760.18197330594956895No Hit
TGTGGG49470.18091277020348023No Hit
GGGGTT48920.17890140930572576No Hit
GGGTAA48920.17890140930572576No Hit
TGGGGG47640.17422042394367895No Hit
CGGGCT46320.16939315778906822No Hit
CTTAGG46070.16847890283554345No Hit
GGGGGT45990.1681863412504155No Hit
AGATTG45670.16701609490990382No Hit
GGCTAG45660.16697952471176283No Hit
GTCTGG45010.16460246183259847No Hit
GTGCGG44820.16390762806791964No Hit
ATAGGG44250.16182312677388316No Hit
TTTGCG44200.16164027578317822No Hit
TGGGAT44010.1609454420184994No Hit
AGATGG43670.15970205528170572No Hit
TGGTTT43660.15966548508356473No Hit
GTTGAT43640.15959234468728276No Hit
AGCGCG43330.15845866854491206No Hit
CTGGTA41690.15246115604978958No Hit
GGCAAC41640.15227830505908463No Hit
CAAATG41210.15070578653902206No Hit
CAGGCG41030.15004752297248422No Hit
GTCCGG41010.14997438257620224No Hit
GGCCAG40130.14675620513979507No Hit
GTCGTC39960.14613451177139822No Hit
GGGCGG39840.14569566939370635No Hit
GTTTCT39820.14562252899742437No Hit
TTAGGC39790.1455128184030014No Hit
CGGGCA39690.1451471164215915No Hit
CCAGGC39640.14496426543088653No Hit
GCCGCG39640.14496426543088653No Hit
TAGCTC39440.14423286146806671No Hit
GGGGAT39250.1435380277033879No Hit
CGAGGG39220.14342831710896495No Hit
CGGGGG39170.14324546611825997No Hit
GCCGTG38470.14068555224839063No Hit
GCTGGA38390.14039299066326272No Hit
AACATG38080.13925931452089202No Hit
GCGGTG37730.13797935758595734No Hit
GGGAGA37710.13790621718967536No Hit
CGCGGG36680.1341394867811533No Hit
TTGTGT36470.13337151262019253No Hit
GTGGGG36440.13326180202576954No Hit
TTTGGG36240.13253039806294975No Hit
GACGGA35920.13136015172243803No Hit
TCGGAA35450.12964135240981148No Hit
AGTGTG35210.1287636676544277No Hit
GTTTTC34740.12704486834180115No Hit
TTGCAT34700.1268985875492372No Hit
GCCTGG34460.12602090279385342No Hit
AGGGTC34270.1253260690291746No Hit
TAGGGG34030.12444838427379083No Hit
CGAGAA34010.12437524387750887No Hit
GGTGGA34010.12437524387750887No Hit
AGGGGT34010.12437524387750887No Hit
GGTGGG33940.12411925249052193No Hit
GGGGCG33790.12357069951840706No Hit
CAGGGG33620.12294900615001023No Hit
TGACTC33450.12232731278161338No Hit
GTGGTG33350.12196161080020348No Hit
TGACGG33350.12196161080020348No Hit
CGGGTG33260.12163247901693457No Hit
GGCTCG33000.12068165386526881No Hit
GCGGGG32730.11969425851546206No Hit
TGAGGG32660.11943826712847512No Hit
GGCGGG32580.11914570554334719No Hit
GGGAAT32380.11841430158052739No Hit
TTCAGA32150.1175731870232846No Hit
TAAGTG32030.11713434464559272No Hit
GGGGTG32020.11709777444745173No Hit
GGACTT31940.11680521286232382No Hit
GCCGAA31890.11662236187161887No Hit
AGGGCG31860.11651265127719589No Hit
ATAAGG31830.1164029406827729No Hit
GTGGAT31820.11636637048463193No Hit
CTACGG31810.11632980028649094No Hit
GCTACG31710.11596409830508102No Hit
GAGTGG31630.1156715367199531No Hit
TTTGCC31490.11515955394597924No Hit
TTATGC31430.11494013275713329No Hit
GCAATG31350.11464757117200536No Hit
TAGTGT31200.1140990181998905No Hit
GGGCAT31200.1140990181998905No Hit
GAGCGG31110.1137698864166216No Hit
AGGGGG31070.11362360562405763No Hit
CGAGAT31010.11340418443521168No Hit
GGGTGC30670.11216079769841801No Hit
TGACTG30530.11164881492444413No Hit
TTCGGG30460.11139282353745722No Hit
GGTCCC30420.11124654274489326No Hit
CTGGGG30330.11091741096162433No Hit
GGAAGG30260.11066141957463739No Hit
TATGCT30250.1106248493764964No Hit
TGGTGG30020.10978373481925362No Hit
ATCGAC29810.10901576065829283No Hit
AGGAGG29670.10850377788431895No Hit
GGAGTG29650.10843063748803697No Hit
CATGGG29380.10744324213823023No Hit
AGGGAG29310.10718725075124329No Hit
AGTTGT29210.1068215487698334No Hit
AGTCCA29150.10660212758098744No Hit
GCTATT28900.10568787262746268No Hit
CCCGTT28890.10565130242932169No Hit
CACTGG28840.10546845143861673No Hit
AGTTGG28790.10528560044791178No Hit
GCGCGG28780.1052490302497708No Hit
TGTGTG28710.10499303886278386No Hit
AGACAG28660.10481018787207891No Hit
TGGGGA28640.10473704747579692No Hit
GGGATT28570.10448105608881No Hit
GCGTAG28500.10422506470182306No Hit
CGCGGA28480.10415192430554107No Hit
GGCGGA28480.10415192430554107No Hit
AGGAAG28300.10349366073900326No Hit
GGGTGT28290.10345709054086225No Hit
AGATGA28260.10334737994643929No Hit
GGCCGG28220.10320109915387533No Hit
TGCACA28200.10312795875759334No Hit
TGGGGT28160.10298167796502938No Hit
GGCAGG28070.10265254618176047No Hit
TGAAAG28010.10243312499291451No Hit
GAGGTA28000.10239655479477353No Hit
GTACGA27980.10232341439849156No Hit
GGAGGG27940.1021771336059276No Hit
GGGAGG27930.10214056340778659No Hit
TCCGCG27910.10206742301150462No Hit
TAACGG27750.10148229984124878No Hit
GGGTTG27640.10108002766169788No Hit
GGTGTG27630.10104345746355689No Hit
TGGTTG27520.100641185284006No Hit
AATGGG27510.100604615085865No Hit
ATGGCG27450.10038519389701905No Hit
CGGGCG27370.10009263231189112No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)