FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483772

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483772
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13627945
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT12300729.026100413525297Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAA275140.20189397594428216No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGCAAAA231270.16970276883271834No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAT194970.1430663243797946No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGCATTT173590.1273779722474665No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAGA159130.11676742164721093No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAG150140.11017068237360805No Hit
TTTGCACATCTTTTTTTCCCATTTTGTCATTCGTCAGATCGGAAGAGCAC145300.10661915644655155No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGGAAA143130.10502684007016466No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGCTTTT138850.10188623449830476No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCAA3379700.0140.883771
CTGCAAT3449100.0137.512362
ACCGCAA476400.0135.929861
CCGCAAT477600.0135.214972
TGCAATT4311950.0126.6036763
CGCAATT556950.0126.493183
CGGCAAT59250.0114.917242
TATGCAA151850.0100.364571
ATTGCAA194000.098.273151
GCAATTT6409600.096.883814
CAATTTC6422050.096.6427155
AATTTCC6415750.096.446466
ACGGCAA70900.096.401891
TTTCCAC6391950.096.057588
ATTTCCA6458100.095.499877
TTCCACA6447750.095.38489
CATGCAA155900.084.838021
GGCAATT86300.082.013183
ATGCAAT564500.079.128552
AATGCAA243750.069.2065661
Produced by FastQC (version 0.11.5)