FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005483781

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483781
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2549817
Sequences flagged as poor quality0
Sequence length6
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGG110330.43269771909121324No Hit
GGGGGA80030.3138656617318027No Hit
GGGTGG79710.31261066970688484No Hit
CGGGGG68170.2673525198082843No Hit
AGGGGG68150.26727408280672693No Hit
GGGCGG66480.2607245931766868No Hit
GGCGGG64940.25468494405676956No Hit
GGGAAG62690.24586078138156583No Hit
CGTAGG62360.24456657085586928No Hit
CCGGGG59820.2346050716580837No Hit
GCGGGG58250.22844776703583042No Hit
GGGCTG57120.22401607644783916No Hit
GGGGGC56130.22013344487074954No Hit
GGGGTG54080.21209365221111945No Hit
TGGGTG53440.2095836681612837No Hit
TGGGGG52840.20723055811456273No Hit
TGGCGG52770.20695602860911194No Hit
GAGGTG52120.20440682605849755No Hit
GGTAGG50820.1993084209572687No Hit
GGGGTC50240.19703374791210504No Hit
AGTTAC49580.19444532686071198No Hit
CGCGGG48410.18985676226960602No Hit
GAGGAG48280.18934692175948314No Hit
GGGGCG47770.18734677821977028No Hit
GATGGG46160.18103259959440227No Hit
GTGTGC45700.17922854855858283No Hit
GGGTGT45030.17660090900641104No Hit
CGGTGG44790.17565966498772265No Hit
TGTCGT44770.17558122798616527No Hit
GGCTGG44410.17416936195813268No Hit
AGGTCA43770.17165937790829697No Hit
ACGCAG43640.17114953739817407No Hit
GAGGGG43530.17071813388960855No Hit
GGGGCT43040.16879642735145306No Hit
GGGCCG43040.16879642735145306No Hit
TTCGGG43010.168678771849117No Hit
GTGCGT43000.16863955334833833No Hit
CGGCCA42650.16726690582108442No Hit
GGGTTG42570.16695315781485495No Hit
GGGGAG41400.162364593223749No Hit
TGGATA41230.1616978787105114No Hit
GGGGAA40710.15965851667001985No Hit
GGGAGG40530.15895258365600354No Hit
TGGGCG40350.15824665064198723No Hit
AGGGGA40150.1574622806264136No Hit
GATCTG39920.15656025510850385No Hit
GGCGGC39740.15585432209448755No Hit
GGCTCG39220.15381496005399603No Hit
GAAGGG39170.15361886755010262No Hit
GCGGTG39140.15350121204776657No Hit
GGCAGA39140.15350121204776657No Hit
TGCGGT39100.15334433804465183No Hit
GCGTTT39010.1529913715376437No Hit
GTGCAG38930.15267762353141423No Hit
GGCAGG38390.15055982448936533No Hit
CGTCTG38340.1503637319854719No Hit
GGAGGG38240.14997154697768506No Hit
GCGAAG38110.14946170646756218No Hit
TGACCT37880.14855968094965247No Hit
CTGGGG37820.14832436994498036No Hit
GGTTGG37630.14757921843018537No Hit
GGGGTA37490.14703015941928382No Hit
GGAGAG37450.14687328541616906No Hit
TGGGGC37340.14644188190760357No Hit
TGTAGG37160.14573594889358726No Hit
GCGCGG36550.14334362034608758No Hit
AGGGGC36440.14291221683752206No Hit
GGTCGG36300.14236315782662048No Hit
CTTCGG36190.141931754318055No Hit
GGGTAA36160.14181409881571894No Hit
CAGGGG36070.14146113230871077No Hit
GCCAGG36030.14130425830559604No Hit
TACGTG35790.14036301428690764No Hit
GTCAGG35700.1400100477798995No Hit
GAGCAG35590.13957864427133398No Hit
TCAGGG35560.13946098876899793No Hit
GTGTGG35450.1390295852604324No Hit
TGCCGG35410.13887271125731768No Hit
GTGGAG35330.1385589632510882No Hit
GGGGAT35190.13800990424018664No Hit
GCCGAG34920.1369510047191622No Hit
GGAGCA34880.13679413071604746No Hit
GGAAGG34570.13557835719190828No Hit
ATAGAG34490.1352646091856788No Hit
ACGGGG34210.1341664911638757No Hit
GCGCCG33990.13330368414674465No Hit
TCACAG33740.13232322162727755No Hit
CCGGAG33730.13228400312649888No Hit
GAGCCG33670.13204869212182677No Hit
GGCCGG33530.13149963311092522No Hit
CAGTGG33460.13122510360547443No Hit
CGGCGG33340.13075448159613023No Hit
GGAGGA33330.13071526309535156No Hit
CGGGAC32920.12910730456342553No Hit
GTGGTT32730.12836215304863055No Hit
GGGCCA32670.12812684204395847No Hit
AATGGG32620.12793074954006503No Hit
GGTTTG32420.12714637952449134No Hit
TTTGGG32340.12683263151826188No Hit
GGATTG32140.12604826150268825No Hit
GGGACG32090.1258521689987948No Hit
GGGGGT32020.12557763949334402No Hit
AGTCAG31890.12506779898322115No Hit
GGCGCG31430.1232637479474017No Hit
CCCAAG31350.12294999994117224No Hit
GCGAGG31270.12263625193494278No Hit
GGTGGC31250.12255781493338541No Hit
GAGGGA31050.12177344491781175No Hit
CGCATG30930.12130282290846754No Hit
GAGATT30890.12114594890535281No Hit
ATGGCG30820.12087141939990202No Hit
GCATTC30510.11965564587576286No Hit
GGGCAT30270.11871440185707445No Hit
GCGGGC30220.11851830935318104No Hit
AGCGCG30150.11824377984773024No Hit
CATGAC30120.1181261243453942No Hit
TACGGA29930.11738097283059921No Hit
GTGGTA29690.11643972881191082No Hit
CGCCGG29450.11549848479322242No Hit
CGTGGG29370.11518473678699295No Hit
CGGTTG29370.11518473678699295No Hit
GCACTC29190.11447880377297665No Hit
AGGAGA29120.11420427426752587No Hit
GAATAC29110.11416505576674718No Hit
AAGGTT29040.11389052626129641No Hit
GGGAGT28960.11357677825506694No Hit
GGATGG28860.1131845932472801No Hit
AGTCGG28760.11279240823949327No Hit
ATGATG28670.11243944173248513No Hit
GTCAAG28580.11208647522547696No Hit
GCCCGG28570.11204725672469829No Hit
TGGCGC28560.11200803822391958No Hit
AAGGAT28490.11173350871846882No Hit
TGGAGT28420.11145897921301803No Hit
ATGAAG28360.11122366820834594No Hit
ATTCGG28300.11098835720367384No Hit
GCCTGG28210.11063539069666567No Hit
ATCGGG27910.10945883567330518No Hit
TGCTGG27820.10910586916629704No Hit
CGGCGC27770.10890977666240362No Hit
GCGGAT27710.10867446565773152No Hit
ATGCCG27700.10863524715695283No Hit
CGGCCG27670.10851759165461677No Hit
TAGGCA27660.10847837315383811No Hit
TTGGGG27510.10789009564215785No Hit
CGAAGG27360.1073018181304776No Hit
CTGGCG27360.1073018181304776No Hit
CCGGTG27350.10726259962969892No Hit
GCCTTA27320.10714494412736286No Hit
CTGCCG27300.1070665071258055No Hit
CACCTG27300.1070665071258055No Hit
AGGAAG27290.10702728862502682No Hit
GGGTAT27220.10675275911957603No Hit
CGGGTG26810.10514480058765002No Hit
CATTGG26810.10514480058765002No Hit
AGGTCG26810.10514480058765002No Hit
AGGCGC26780.10502714508531397No Hit
TTGTTA26700.1047133970790845No Hit
AACCGG26700.1047133970790845No Hit
CAGGCA26660.10455652307596976No Hit
GGGGAC26650.1045173045751911No Hit
TGGGAC26600.10432121207129767No Hit
CGGGTA26560.10416433806818293No Hit
GTGGGT26460.10377215306039611No Hit
TGTGGC26310.10318387554871584No Hit
GGTAAA26300.10314465704793717No Hit
GTCCCG26250.10294856454404376No Hit
GAGTGT26190.10271325353937164No Hit
TTCTGG26050.10216419452847007No Hit
GGCCTG26030.10208575752691271No Hit
TACAGG26010.10200732052535535No Hit
CGAGGG26000.10196810202457665No Hit
GAAATG25940.10173279101990457No Hit
CACCAG25900.10157591701678984No Hit
AAATCA25860.1014190430136751No Hit
AGCCGT25840.10134060601211772No Hit
GGCACG25810.10122295050978168No Hit
TCGCCG25730.10090920250355222No Hit
CGGTCG25670.10067389149888012No Hit
CAACGG25520.10008561398719985No Hit
AGATCC25500.10000717698564252No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)