FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483786

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483786
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12839495
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT5957064.639637306607463Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT906100.0126.153352
ACTGCAA943100.0121.652661
TGCAATT1058300.0118.1235663
CGCAATT142100.0117.36263
CCGCAAT137500.0115.899652
ACCGCAA145400.0109.930511
GCAATTT1443750.098.856664
CAATTTC1480650.096.383195
AATTTCC1479150.096.141316
TTTCCAC1494700.094.5760658
TTCCACA1530700.092.455939
ATTTCCA1546750.091.74677
CGGCAAT32100.046.181852
TCCGATC52050.039.9281925
ATTGCAA110500.037.6433871
CGGAAGT50950.036.368915
CCGATCT57250.035.8307886
TTCCGAT58400.035.2133944
ACGGCAA44250.034.182591
ATGCAAT187300.032.4350622
Produced by FastQC (version 0.11.5)