FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483794

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483794
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13379335
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT4429333.3105756003568185Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT982650.0125.7284242
TGCAATT1117900.0121.2068943
ACTGCAA1024950.0121.0989151
CGCAATT142950.0119.137423
CCGCAAT139650.0115.711112
ACCGCAA145900.0111.130571
GCAATTT1485500.0103.625894
CAATTTC1533450.0100.389945
AATTTCC1539500.099.620096
TTTCCAC1556100.097.612828
TTCCACA1575900.096.699149
ATTTCCA1606900.095.191037
CGGCAAT33250.054.411282
TCCGATC55050.044.6096955
ATGCAAT180600.042.886512
CCGATCT57850.042.5997476
TTCCGAT57850.041.9483224
ACGGCAA46600.038.850571
ATTGCAA118750.032.0834921
CTTCCGA81550.029.8466473
Produced by FastQC (version 0.11.5)