FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483796

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483796
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6600303
Sequences flagged as poor quality0
Sequence length151
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT214834932.54924811785156Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTC213030.3227579097505069Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT122040.1849006022905312Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC116920.17714338265985669RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGGAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC116670.17676461217007766RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGT112080.16981038597773465Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
CCCCCAGTCCACTGTCTCTGGGCCCAGTTTAGACCTAGGACTCAAGATCG92910.14076626482147866No Hit
AGATCGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC85970.13025159602521277Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAA77810.11788852723882526No Hit
CTCTTCCGATCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTC69430.10519214042143217Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGCAAAA68110.1031922322353989No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCAA1023550.0136.91081
CTGCAAT1021950.0136.502322
TGCAATT1220350.0127.653823
CCGCAAT140200.0127.600892
CGCAATT156350.0121.3916853
ACCGCAA166550.0107.832511
GCAATTT1702550.0103.573294
CAATTTC1710200.0103.0462655
AATTTCC1708750.0102.7764056
TTTCCAC1697300.0102.706488
ATTTCCA1719650.0101.840247
TTCCACA1720950.0101.375079
CGGCAAT19750.088.667322
ATTGCAA69950.084.612421
GGCAATT27750.065.724063
TCGGAAG5553500.064.467124
CGGAAGA5527450.064.323985
ATCGGAA5606150.064.28813
GATCGGA5628650.064.115032
AGATCGG5649400.063.9534651
Produced by FastQC (version 0.11.5)