FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005483799

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483799
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8603836
Sequences flagged as poor quality0
Sequence length6
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTG248460.288778168249604No Hit
GGTTGG199880.23231498136412643No Hit
GCGGTG189910.2207271268304045No Hit
TGTGTG182420.21202170752673577No Hit
GTGGCG177750.20659389602498235No Hit
GGGGGT175760.20428097420731867No Hit
CCGGGG175620.2041182560894931No Hit
GGGGGG168190.19548257312203532No Hit
GTGGGG165470.19232119254713825No Hit
CGTGTG164880.19163545190773046No Hit
GGGGCG164470.19115892027695552No Hit
GGAGGA160940.18705609916321045No Hit
TTGGGG158430.18413879576505177No Hit
GGTGGG156360.18173289216577349No Hit
TTTCGG153260.17812984812820698No Hit
GTGGTG152310.17702568947153338No Hit
GTACGG151650.1762585897732128No Hit
GTTGTT151550.1761423625461945No Hit
TGCTTT151170.17570069908352506No Hit
GTGGGT146290.1700288104050333No Hit
GGGGGA145170.16872706546242863No Hit
AGGCCG143390.16665822082150333No Hit
GTGGGC135120.15704622914709207No Hit
GTTGGT134720.15658132023901897No Hit
CGGTGG130470.1516416630907423No Hit
GGAGTG129710.15075833616540343No Hit
GGGGAG129270.15024693636652303No Hit
GGGGGC129220.1501888227530139No Hit
CGTGGG128870.14978202745844993No Hit
GGGTGG126140.14660902416085106No Hit
GGGTTG125960.14639981515221814No Hit
GGTGGC125760.14616736069818162No Hit
TTGTGG125520.14588841535333774No Hit
GGAGGG125140.1454467518906683No Hit
GGAGAA124100.14423798872967825No Hit
AGGATG122880.1428200165600553No Hit
GTATAT122490.14236673037468403No Hit
TGGGTT122450.14232023948387673No Hit
GGCAGT121860.1416344988444689No Hit
GGTAGG118880.13817092747932433No Hit
GTGTGT117510.136578614469174No Hit
GGAGAG116730.13567204209843145No Hit
TGGGAC115380.13410297453368475No Hit
GCGGGG115000.1336613110710153No Hit
GGGCCT114340.1328942113726947No Hit
GTCTGT112550.1308137440090676No Hit
TGGGGC112530.13079049856366393No Hit
GATGGT112490.13074400767285663No Hit
GGAGGT110410.12832648135087651No Hit
CTGGTG108870.1265365820547951No Hit
CGGGGG108780.12643197755047866No Hit
GGGTAT108080.12561838696135072No Hit
CAGGCC107280.12468856914520453No Hit
GGCGGG106160.12338682420259986No Hit
GTATGG105590.1227243290085957No Hit
TGTGGT105460.12257323361347194No Hit
GTTCGT105170.12223617465511895No Hit
GATTGG104050.12093442971251428No Hit
ATTGTT102850.11953970298829499No Hit
TGCGGG102570.11921426675264382No Hit
TGTATA101590.11807523992786474No Hit
GTAGGT101390.11784278547382818No Hit
AGGTTC101230.11765682191059894No Hit
GTAAGT100640.11697108127119113No Hit
GTTCGG100510.11681998587606737No Hit
GTCGGG100410.11670375864904911No Hit
CTGGGG99720.115901790782623No Hit
CTGCAG99650.11582043172371022No Hit
GTTATA98810.11484412301675671No Hit
GGGCTG97650.11349588718334473No Hit
GTAATT97610.11344939629253743No Hit
GGCTGG95540.11104349269325914No Hit
GCAGGG95380.11085752913002991No Hit
GGTGGT95230.11068318828950251No Hit
GGGGAC95210.11065994284409884No Hit
GGGTGC94900.11029963844034218No Hit
TGAGGG94840.11022990210413124No Hit
GTGGAG94400.1097185023052508No Hit
ACGGGG94180.10946280240581062No Hit
CTCGGG93870.10910249800205396No Hit
TTTTCG93720.10892815716152657No Hit
TTGGTG93720.10892815716152657No Hit
ATTAGG93040.10813781201780229No Hit
TCCTTG92950.10803320751348584No Hit
GGGAGT92630.10766128038702738No Hit
AGGGGG92430.10742882593299082No Hit
GTGCGG92260.10723123964705976No Hit
GATGTA91880.10678957618439032No Hit
GAGAAG91190.10598760831796422No Hit
ATTGCC91180.10597598559526239No Hit
GAAGAG91160.10595274014985874No Hit
GGCGGA90720.10544134035097832No Hit
GTTGTG90590.10529024495585457No Hit
TTGTTT90350.10501129961101072No Hit
GGGTCA90170.10480209060237783No Hit
TGGGGT90040.10465099520725407No Hit
TTAAGA89890.10447665436672665No Hit
GGGAAT89440.10395363184514442No Hit
CAGGCT89390.10389551823163529No Hit
ATGATG89330.10382578189542432No Hit
GGGTCG88870.10329113665114027No Hit
AAGGGC88870.10329113665114027No Hit
TAGGGT88800.10320977759222746No Hit
CTTTGG88750.10315166397871832No Hit
GAGTGG88660.10304705947440189No Hit
GCGGGT88500.10286109591117264No Hit
CGCGGG88230.1025472823982233No Hit
TTAGGA88030.10231482794418677No Hit
TTCTGG87940.10221022343987031No Hit
TAGGTA86650.10071089221133459No Hit
TTGTTG86590.10064115587512361No Hit
TGGTGG86130.10010651063083954No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)