FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483802

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483802
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2833502
Sequences flagged as poor quality0
Sequence length151
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT46108216.272513659775075Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTC84520.29828812543629757Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAA77150.27227790910329336No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGCAAAA67030.23656238816842198No Hit
AATACTAGTTGGCTTGTAGCTCCTGCAATTTCCACAGGGGTAGTCGATCC65480.2310921255746423No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAT55880.19721178950994211No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGCATTT50150.17698946392132422No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAGA45310.1599081278220379No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAG41790.14748533793164784No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGGAAA40200.14187390727093185No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGCTTTT39830.14056810265177155No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGCAAAG38170.13470962787391716No Hit
AGATCGGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGT37270.13153334636785152Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAGAA36220.12782768461077493No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAC36220.12782768461077493No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGCCAGG35000.12352205856921929No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAACA34680.12239271403372928No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGCAAAT33920.11971052076194054No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGGAGG33430.11798121194197145No Hit
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT31970.1128285774987983Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAGG31700.11187569304697861No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGCAACA31640.11166394094657424No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGGAGA31530.11127572876249954No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGCAGA31420.11088751657842486No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAGGG31150.10993463212660518No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAATT31100.10975817204293487No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGCCTTT30960.10926408380865797No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGGGGG29870.10541725398464516No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGGAAG29660.10467612163322984No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGCAAGA29530.10421732541568703No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGCCAAA29010.10238214054551575No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGGAAT28700.10128808802675983No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAGT28650.10111162794308952No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCAA976150.0143.154281
CTGCAAT988300.0140.532322
CCGCAAT135150.0138.121582
ACCGCAA139000.0134.747651
TGCAATT1193850.0130.393693
CGCAATT152500.0130.028763
CGGCAAT16000.0121.710562
TACTAGT11950.0111.272793
ATTGCAA48300.0105.074771
GGCAATT20250.0102.264673
GCAATTT1743750.0101.536834
CAATTTC1747650.0101.247885
AATTTCC1746850.0100.869136
TTTCCAC1738700.0100.6827248
ATTTCCA1750400.0100.4291157
TTCCACA1750450.0100.300869
ACGGCAA19650.099.9059451
TATGCAA25200.099.5425341
CTAGTTG13900.095.6625755
ACTAGTT14050.094.6412664
Produced by FastQC (version 0.11.5)