FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483803

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483803
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2833502
Sequences flagged as poor quality0
Sequence length6
%GC61

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGGG168280.5938940576008064No Hit
GGGGGG155400.5484379400473337No Hit
GGGGAC131380.46366651585211516No Hit
GGGGGT127990.4517025221792679No Hit
GGGTGG122240.43140961255718185No Hit
GGGCGG121810.4298920558376172No Hit
GGCCGG108480.3828477975311117No Hit
GCGGGG102840.3629431000931003No Hit
GTGGGC99810.35224961902267937No Hit
GGAACA94830.3346741946891162No Hit
CCTGGG90230.31843986699144733No Hit
GGCTGG88280.3115579237283051No Hit
TTGCGG86860.3065464573520682No Hit
GTGGTA86730.3060876611345254No Hit
GCGGGA85370.30128794685869287No Hit
GGGGCG84970.2998762661893304No Hit
GGAAAA84280.29744111703468007No Hit
TAGAGG83230.2937354552776035No Hit
TGGAGG82850.2923943586417091No Hit
GGAGGA82120.2898180414201225No Hit
GGGTAG77500.27351312968898556No Hit
GTGAGG76230.2690310435637596No Hit
ATCTCG73010.25766701417539145No Hit
GAGGCT72450.25569066123828393No Hit
CTGGCG71180.251208575113058No Hit
GTGGCG70620.24923222217595048No Hit
AGGGCG70500.24880871797514173No Hit
CTAGGG70100.2473970373057792No Hit
CGGGGG69040.24365608353196858No Hit
TCGAGC68930.24326787134789388No Hit
GGCGTG68590.24206794277893576No Hit
GGGCAG67720.23899753732307233No Hit
TGGGGC67400.23786819278758228No Hit
GGTGCT66710.235433043632932No Hit
GGCAGC66380.23426840708070792No Hit
GGGCCG66080.233209646578686No Hit
GGGCCA65760.23208030204319602No Hit
GTGGGG65460.23102154154117416No Hit
GTGCGC65310.2304921612901632No Hit
AGGTGG63960.22572773903106474No Hit
GGGTGC62370.22011630837034876No Hit
TGGTGG62230.2196222201360719No Hit
GTGGAG61690.2177164512324325No Hit
CCGTTA60380.2130931970402703No Hit
AGGGTA59670.21058746385215185No Hit
ACGAGG59210.208964031082385No Hit
TCGGCG57570.2031761403379987No Hit
GTTTGA57460.20278792815392402No Hit
GGGCGC57120.20158799958496587No Hit
TGCGCG57070.2014115395012956No Hit
AGGTTT56830.2005645310996781No Hit
GATGGA56690.20007044286540118No Hit
GGACAC56380.19897639034664524No Hit
GCAGGT55680.19650594917526085No Hit
GGGCGT55150.19463547228835554No Hit
TCTGTG54500.19234149120064145No Hit
GATCTG54300.1916356508659602No Hit
GGTGGG54140.19107097859821523No Hit
ACGAAG53380.18838878532642644No Hit
GCGGGT52470.18517721180362673No Hit
GGGGCC51980.18344790298365768No Hit
GGTCGT51880.18309498281631706No Hit
GGGGGA51790.1827773546657105No Hit
GACCGG51740.18260089458204015No Hit
GCCGGT51210.18073041769513487No Hit
GTGGAA51200.1806951256784008No Hit
TTGTGG51140.1804833735779964No Hit
GGGTTC50970.17988340929351734No Hit
GTGCCC50660.1787893567747614No Hit
GAGCGG50340.1776600122392714No Hit
GGCGTA50140.17695417190459015No Hit
CGTGGG50120.17688358787112202No Hit
GCGGTG49840.17589541140256826No Hit
GAGGGG49530.17480135888381232No Hit
ATGGCA49150.17346026224791797No Hit
GGCCCG49070.17317792611404545No Hit
GTCTGG48910.17261325384630044No Hit
ATGGAA48690.17183682947815107No Hit
GTGTGG48300.1704604408255226No Hit
GGCAGG48070.1696487244406392No Hit
AGTATT47930.16915463620636229No Hit
TGGGGG47750.16851937990514917No Hit
GCGGCG47360.16714299125252075No Hit
GGGGGC47270.16682536310191418No Hit
TGCCTT47190.16654302696804169No Hit
TGGGTT47130.1663312748676373No Hit
AGCTGC47050.16604893873376478No Hit
CGCGGG46940.1656607265496901No Hit
GGCAAG46940.1656607265496901No Hit
AAGCGA46270.1632961614285079No Hit
GGTGTC46240.1631902853783057No Hit
GGGAGT46020.16241386101015634No Hit
GGACGG45970.162237400926486No Hit
GTGCGG45910.16202564882608164No Hit
GGGGTA45830.16174331269220915No Hit
AAGGTA45660.1611433484077301No Hit
ATAGGG45600.1609315963073257No Hit
AAGTTG45510.16061396815671916No Hit
CAGCGC45500.16057867613998508No Hit
AGATTG45410.1602610479893785No Hit
TACTGC45340.16001400387224007No Hit
ACTACG45170.15941403958776101No Hit
GGGAGA45080.15909641143715444No Hit
TGTGGT44940.15860232320287757No Hit
ACGATC44920.15853173916940944No Hit
CAGGGT44890.15842586311920726No Hit
GTGACG44850.158284695052271No Hit
GAGGTG44640.15754356270085568No Hit
GGAGGG44470.15694359841637662No Hit
AGCGGC44450.15687301438290852No Hit
GGAGGT44400.15669655429923818No Hit
GCGAAC44360.15655538623230192No Hit
ACTCGT44100.15563779379721632No Hit
GAGTAC43950.15510841354620536No Hit
ACGGGG43930.15503782951273726No Hit
GGATTT43750.15440257321152412No Hit
GGCACT43750.15440257321152412No Hit
AAACTG43700.1542261131278538No Hit
CACGGT43640.15401436102744942No Hit
GGGTCG43640.15401436102744942No Hit
GGTGGT43610.15390848497724724No Hit
GTTGGG43490.1534849807764385No Hit
GATCAT43310.15284972447522535No Hit
GAGACT43200.15246151229115068No Hit
CGCCGG43060.15196742405687377No Hit
AGCAGG43010.15179096397320346No Hit
TGTGGC42970.15164979590626723No Hit
GAAGCT42970.15164979590626723No Hit
GGTGAG42910.15143804380586284No Hit
GGGTTA42900.1514027517891288No Hit
TATCTA42810.15108512363852222No Hit
AGGTCG42680.1506263274209794No Hit
AATTTG42630.15044986733730908No Hit
TCTACA42450.14981461103609597No Hit
CCCTGG42440.1497793190193619No Hit
GCACAG42340.14942639885202127No Hit
TAAAAG42070.1484735144002016No Hit
TCGTGG42010.1482617622997972No Hit
GAGTGC41800.1475206299483819No Hit
GTCGGT41780.14745004591491376No Hit
AGGGTG41750.14734416986471158No Hit
GTGGTG41680.14709712574757314No Hit
CGAGTA41570.14670891356349847No Hit
CGGTAG41510.14649716146309408No Hit
CTCGGT41430.14621482532922156No Hit
GTATCT41380.14603836524555125No Hit
CACGTA41350.14593248919534907No Hit
TTTGTC41150.1452266488606678No Hit
ATGAGG41090.14501489676026347No Hit
GCGCGG41030.14480314465985908No Hit
AGGTTG41010.14473256062639095No Hit
CCAGGG40950.14452080852598656No Hit
CATTTT40830.1440973043251778No Hit
GCTGAG40660.14349734004069875No Hit
GGTTAG40610.14332087995702844No Hit
CAGCAA40470.14282679172275156No Hit
GGTCGA40420.14265033163908125No Hit
AGAACG40420.14265033163908125No Hit
GGGACG40180.14180332323746375No Hit
GGTGCC40170.14176803122072967No Hit
AAGTTA40170.14176803122072967No Hit
ATGGCG40130.14162686315379344No Hit
CGGGAG40050.14134452701992092No Hit
CTGTCC39900.14081514676891No Hit
GTTCGG39880.14074456273544186No Hit
GAGAAC39690.14007401441749467No Hit
GTTTAG39590.13972109425015405No Hit
GGGTCT39500.13940346609954749No Hit
GCGACT39490.13936817408281343No Hit
GTCATG39330.13880350181506843No Hit
GGGAGG39110.13802707744691906No Hit
GCCGTA39070.1378859093799828No Hit
GGCGGG39050.13781532534651467No Hit
CCGGCA38930.13739182114570592No Hit
GGCGGA38910.1373212371122378No Hit
GACTGC38910.1373212371122378No Hit
GAGGAT38860.13714477702856748No Hit
AGGGGC38830.1370389009783653No Hit
GGGAGC38760.13679185686122686No Hit
GCTGCG38480.1358036803926731No Hit
AGGCCG38270.13506254804125778No Hit
GACTTG38080.1343919997233106No Hit
GGAGTT38020.1341802476229062No Hit
TCGATC37860.13361557535516122No Hit
TGAATT37680.13298031905394808No Hit
GGGTTG37540.1324862308196712No Hit
GTGCCA37500.13234506275273497No Hit
TAATAC37470.13223918670253276No Hit
GCGTAT37430.13209801863559653No Hit
CTGGGC37410.13202743460212837No Hit
AGTCGC37370.13188626653519214No Hit
TCAAGA37240.13142747031764931No Hit
AGAGGG37240.13142747031764931No Hit
GTGCCG37240.13142747031764931No Hit
CCAGAA37170.1311804262005109No Hit
ACGCGG37150.13110984216704277No Hit
GTCAAT37140.1310745501503087No Hit
TTTGGC37100.13093338208337246No Hit
ATTGGA37010.13061575393276587No Hit
GATACA36950.1304040018323615No Hit
GGGCTG36950.1304040018323615No Hit
ATTACC36850.13005108166502088No Hit
GTTGTG36720.12959228544747808No Hit
GCCGAC36710.129556993430744No Hit
TAAGTA36640.12930994931360557No Hit
AGAGAA36620.12923936528013744No Hit
GGTAAG36510.12885115309606274No Hit
TACGTG36400.12846294091198807No Hit
GTTGGT36340.12825118881158368No Hit
ATGCTG36310.1281453127613815No Hit
ATGATC36170.12765122452710462No Hit
ATGAGA36150.12758064049363652No Hit
TGCGTG35950.12687480015895525No Hit
ATGTGC35870.12659246402508276No Hit
CAGTTA35700.12599249974060367No Hit
GGGTAA35690.12595720772386962No Hit
CTATGG35550.12546311948959274No Hit
GAGGCG35450.12511019932225212No Hit
GCAGAC35380.12486315520511367No Hit
GCGCCG34870.12306326235167649No Hit
ATGAAC34860.12302797033494242No Hit
GCGAAG34700.12246329806719743No Hit
TGCGTC34570.1220045018496546No Hit
CTAGCC34480.12168687369904804No Hit
TCGGGG34250.1208751573141646No Hit
AGGCGT34210.12073398924722833No Hit
GGCGTT34190.12066340521376022No Hit
TAGTAT34070.12023990101295147No Hit
GGCAAC34060.1202046089962174No Hit
TCTAGG33820.11935760059459989No Hit
GGAAGG33690.11889880437705709No Hit
TATGGC33590.11854588420971644No Hit
GCTGTC33520.11829884009257803No Hit
GTTATC33470.11812238000890772No Hit
CGGGGC33460.11808708799217364No Hit
CTGGTT33410.11791062790850333No Hit
ATTGGC33340.11766358379136489No Hit
GGCGGT33310.1175577077411627No Hit
CTCTGG33160.11702832749015177No Hit
GGCACG33120.11688715942321552No Hit
GTAAGG33080.11674599135627928No Hit
GCCGCA33080.11674599135627928No Hit
GCTGCT33010.11649894723914082No Hit
TGAAGG32850.11593427497139583No Hit
TGTACG32720.115475478753853No Hit
AGAAGA32540.11484022245263988No Hit
GGCTGT32370.1142402581681608No Hit
GGGGCT32350.1141696741346927No Hit
TATCGA32290.11395792203428833No Hit
AACGGT32150.11346383380001143No Hit
TCATGG32050.11311091363267081No Hit
ACCTGG32010.11296974556573457No Hit
AAGGGG31980.11286386951553237No Hit
TAGGAT31880.11251094934819174No Hit
TGTAAG31680.1118051090135105No Hit
CCGCGG31580.11145218884616986No Hit
CTGCGC31440.11095810061189298No Hit
TGGGAG31270.11035813632741392No Hit
CGCGTT31190.11007580019354142No Hit
GTTTGC31170.1100052161600733No Hit
AGGAGC31070.10965229599273266No Hit
AGCGGG31070.10965229599273266No Hit
GGTTGG30720.10841707540704049No Hit
ACGTGT30620.10806415523969984No Hit
GTCCCG30530.10774652708909328No Hit
CTGGCT30490.10760535902215704No Hit
ATATGA30290.10689951868747577No Hit
CTTCGG30230.1066877665870714No Hit
CCGGGG30160.10644072246993297No Hit
GGGGTG30100.1062289703695286No Hit
GAGGGC30060.10608780230259234No Hit
GAGGGT30010.10591134221892202No Hit
TACATC29780.10509962583403858No Hit
GCTTGT29360.10361736113120795No Hit
TTCGTT29320.10347619306427171No Hit
CGTGAA29280.10333502499733545No Hit
GTAGTA29200.10305268886346296No Hit
AGCACG29190.10301739684672889No Hit
CAGGGG28940.10213509642837731No Hit
ACCGCG28890.101958636344707No Hit
AAGGAG28890.101958636344707No Hit
TTTGGA28830.10174688424430263No Hit
GCGGAT28800.10164100819410045No Hit
CCATGA28720.10135867206022793No Hit
GCCTAG28700.10128808802675983No Hit
GACGGG28660.10114691995982356No Hit
CAGGTT28660.10114691995982356No Hit
TCAATG28430.10033520357494013No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)