FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483807

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483807
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences766033
Sequences flagged as poor quality0
Sequence length6
%GC60

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGG43570.5687744522755547No Hit
GGGGGT39510.5157741246134305No Hit
GTGGGG38680.5049390822588583No Hit
GCGGGG34260.4472392181537871No Hit
GCCGCG29520.3853619883216519No Hit
CGGGGG28320.369696866845162No Hit
GAGGTG27110.3539012026897014No Hit
GGGTCG25610.33431980084408897No Hit
TGGGGG24990.3262261547479025No Hit
GAGGGG23120.30181467378037236No Hit
AGTTGG22680.2960707959056594No Hit
AGGGGT21930.28628009498285323No Hit
ATCTCG21660.282755442650643No Hit
GGGCGG21420.279622418355345No Hit
TGTGGG21210.2768810220969593No Hit
GGGGGA20940.27335636976474903No Hit
GGGGCA20140.2629129554470891No Hit
GAGCGT19500.25455822399296113No Hit
GGGTGC19380.25299171184531216No Hit
GAGTGG19010.24816163272339442No Hit
GGGCCG18870.24633403521780395No Hit
GATTGG18770.24502860842809646No Hit
GGTGGT18660.2435926389594182No Hit
GGGCGC18510.24163449877485696No Hit
CGGGCT18180.23732659036882223No Hit
GCTAGG17900.23367139535764125No Hit
ACCGCG17830.23275759660484602No Hit
GGTTGG17810.23249651124690449No Hit
GGTGGG17540.22897185891469427No Hit
GGGTGG17440.22766643212498677No Hit
CGTGGG17290.22570829194042555No Hit
GGAGGT17130.22361960907689354No Hit
TCGGGG17060.2227058103240983No Hit
ATGCAA16790.2191811579918881No Hit
AGGGGG16620.21696193244938536No Hit
TGATAT16570.21630921905453157No Hit
GTGTGG16320.21304565208026285No Hit
GGTGGA15910.20769340224246216No Hit
AGAGGG15670.20456037794716417No Hit
GGGAGG15460.2018189816887784No Hit
ACGCCG15240.19894704275142194No Hit
CTGGGG15110.1972499879248022No Hit
TAGGGA15100.19711944524583147No Hit
GGGGCG14950.1951613050612702No Hit
TTGGGG14870.1941169636295042No Hit
GTAGGA14750.1925504514818552No Hit
CGGGGT14600.19059231129729398No Hit
GAAGGG14490.18915634182861574No Hit
GGGGTG14350.18732874432302526No Hit
AAGGCG14340.1871982016440545No Hit
GACCGG14300.1866760309281715No Hit
GAGGCG14260.1861538602122885No Hit
GCGTGT14260.1861538602122885No Hit
GGGGGC14190.18524006145949326No Hit
GACAGG14150.18471789074361028No Hit
TATGCG14140.1845873480646395No Hit
GCCCGC13900.18145432376934154No Hit
ACGGGG13800.18014889697963404No Hit
AGGGGC13790.1800183543006633No Hit
GTTGGG13710.17897401286889728No Hit
CAAGGG13560.17701587268433605No Hit
GGGTAA13390.1747966471418333No Hit
CAGGTG13120.17127199480962307No Hit
ACGGGA13100.17101090945168157No Hit
GGCGTC13070.17061928141476934No Hit
CCTGGG13060.1704887387357986No Hit
AGTGCA12980.1694443973040326No Hit
GCTTTG12880.1681389705143251No Hit
GGGGCT12830.16748625711947135No Hit
GTATGT12800.1670946290825591No Hit
TGCGAG12760.1665724583666761No Hit
CTCGGG12740.1663113730087346No Hit
ACTCAT12660.16526703157696862No Hit
GCGGAG12660.16526703157696862No Hit
TTTGGA12650.16513648889799787No Hit
CAGAAC12550.16383106210829038No Hit
GGGTAT12540.16370051942931962No Hit
GGACGG12480.1629172633554951No Hit
TAGGGC12470.1627867206765244No Hit
GGGTGA12420.16213400728167066No Hit
TTGCCG12350.1612202085288754No Hit
TCCGGT12350.1612202085288754No Hit
GTGGGC12320.16082858049196314No Hit
ATGTTG12300.16056749513402163No Hit
AAGGGG12280.16030640977608013No Hit
AGCAGT12250.15991478173916787No Hit
GTGGGT12250.15991478173916787No Hit
CGGGCG12190.1591315256653434No Hit
TTCGCC12180.15900098298637264No Hit
AGTGCT12180.15900098298637264No Hit
GCGGGT12100.15795664155460667No Hit
GTCTTT11950.15599850137004542No Hit
GGGGAG11930.15573741601210392No Hit
CGAAGC11810.15417090386445492No Hit
ACATAC11780.15377927582754267No Hit
ATAGGC11700.15273493439577668No Hit
GGAGCG11690.15260439171680593No Hit
GACGAG11640.1519516783219522No Hit
GGTGCC11600.15142950760606919No Hit
ATCGAG11490.14999353813739094No Hit
GCGGGA11460.14960191010047869No Hit
TAGCAG11390.14868811134768345No Hit
GCAGGC11370.14842702598974195No Hit
AGGTCC11330.14790485527385897No Hit
GGCGGT11310.14764376991591746No Hit
GCGTTA11270.14712159920003445No Hit
GGAAGG11170.14581617241032696No Hit
GGCAGG11170.14581617241032696No Hit
AGTGGG11120.14516345901547323No Hit
CAGGGC11090.14477183097856097No Hit
GCTTAA10970.14320531883091198No Hit
ACTAGA10900.14229152007811674No Hit
TGGGAG10900.14229152007811674No Hit
GCCGGG10900.14229152007811674No Hit
TTAGAT10830.1413777213253215No Hit
GGACGC10820.14124717864635072No Hit
CCGGGT10790.1408555506094385No Hit
TCGTGG10750.1403333798935555No Hit
ATGAGT10730.140072294535614No Hit
TGACGG10680.13941958114076025No Hit
GGGACG10640.13889741042487727No Hit
CATGGG10610.13850578238796502No Hit
AGGTGG10580.13811415435105276No Hit
TCCAGG10560.13785306899311126No Hit
CCCGTC10530.13746144095619903No Hit
CCGTAC10370.13537275809266702No Hit
AGGGTG10240.1336757032660473No Hit
GTAGGT10230.13354516058707652No Hit
ACGGGT10130.13223973379736903No Hit
TTATCA10120.1321091911183983No Hit
TACTTA10050.13119539236560304No Hit
TAGTAG10010.13067322164972006No Hit
GCGGTG9990.13041213629177856No Hit
TGTGTA9970.13015105093383705No Hit
ATAGCC9960.1300205082548663No Hit
AAGTAC9890.12910670950207107No Hit
GTCATG9810.12806236807030505No Hit
ATAGAC9800.12793182539133433No Hit
AGTTAC9780.12767074003339282No Hit
CGCGGG9760.12740965467545132No Hit
GGGTGT9760.12740965467545132No Hit
GGGTTG9660.12610422788574382No Hit
GCTGAG9650.12597368520677307No Hit
TGGTGG9640.12584314252780232No Hit
CGTGAA9610.12545151449089006No Hit
TTTAAG9600.12532097181191934No Hit
GGTCGG9540.12453771573809483No Hit
GGATGG9520.12427663038015334No Hit
TGGTTT9520.12427663038015334No Hit
GGGAAA9430.1231017462694166No Hit
GAGCGG9430.1231017462694166No Hit
TATGTT9360.12218794751662133No Hit
GGAGTC9360.12218794751662133No Hit
GCTTCT9360.12218794751662133No Hit
GATGGG9360.12218794751662133No Hit
TAGGGG9350.1220574048376506No Hit
TGAGTG9340.12192686215867984No Hit
GACTCG9330.1217963194797091No Hit
CAGGCA9310.12153523412176759No Hit
GCGCTG9280.12114360608485536No Hit
GGCGTT9170.11970763661617712No Hit
GCGACG9140.11931600857926486No Hit
TTGTGA9130.11918546590029412No Hit
TCGGAG9120.11905492322132336No Hit
ATGACA9050.11814112446852812No Hit
AAGGGA9000.11748841107367437No Hit
AGGTAC8950.11683569767882063No Hit
CGCTGG8910.11631352696293763No Hit
CGAAAT8880.11592189892602539No Hit
ATTGGG8880.11592189892602539No Hit
GTCCAG8870.11579135624705462No Hit
GAAGTG8860.11566081356808389No Hit
ATGCCT8830.11526918553117163No Hit
GGGCAA8790.11474701481528862No Hit
GTGCGG8760.1143553867783764No Hit
TCACGG8730.11396375874146414No Hit
GGAAAC8730.11396375874146414No Hit
TTGGTA8710.11370267338352265No Hit
AGGGCG8640.11278887463072738No Hit
CGAGGG8610.11239724659381516No Hit
CGGGCA8590.11213616123587367No Hit
GTGTAT8570.11187507587793215No Hit
CGCGCT8500.1109612771251369No Hit
GGGGTC8500.1109612771251369No Hit
GGGCTC8500.1109612771251369No Hit
AATTCG8500.1109612771251369No Hit
GTAAGA8490.11083073444616617No Hit
TAGAGG8470.11056964908822466No Hit
CGGTAA8430.11004747837234166No Hit
CTGCGT8420.10991693569337091No Hit
GACTTG8380.10939476497748792No Hit
TTTTTG8370.10926422229851715No Hit
GATAGA8320.1086115089036634No Hit
GTATGG8320.1086115089036634No Hit
CGTATA8310.10848096622469268No Hit
CGAAGG8280.10808933818778042No Hit
GGATAG8280.10808933818778042No Hit
GGCGGG8280.10808933818778042No Hit
CGGTGT8260.10782825282983893No Hit
GTAAGG8230.10743662479292668No Hit
GGTAGG8210.10717553943498517No Hit
ATGGTG8200.10704499675601442No Hit
GGAGGG8190.10691445407704368No Hit
TGGGTG8190.10691445407704368No Hit
GGTTTT8190.10691445407704368No Hit
GAGGGT8160.10652282604013143No Hit
CAGTTA8120.10600065532424845No Hit
ACCGTG8080.10547848460836544No Hit
ATGGCG8070.1053479419293947No Hit
GGGGTT8030.10482577121351169No Hit
CTTGGT8010.10456468585557019No Hit
GGCCGG8010.10456468585557019No Hit
CAGGAG7960.10391197246071644No Hit
TCAAAG7940.10365088710277495No Hit
CGCAGC7940.10365088710277495No Hit
GGCGCG7920.10338980174483345No Hit
CGGTCT7920.10338980174483345No Hit
GGGGCC7900.10312871638689194No Hit
GTGTAG7890.1029981737079212No Hit
GGTCGA7890.1029981737079212No Hit
GACTGT7860.10260654567100895No Hit
CGGCCG7850.1024760029920382No Hit
CCTCGC7850.1024760029920382No Hit
GATGTG7820.10208437495512596No Hit
ATGGGG7790.10169274691821371No Hit
TGCCCA7770.10143166156027221No Hit
CCGTGG7750.10117057620233072No Hit
CTTGAG7740.10104003352335997No Hit
AGCGGG7730.1009094908443892No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)