FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483808

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483808
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8191933
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT83394110.180027107155295Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTC114170.1393688156385068Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAA113160.13813589539855856No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGCAAAA100210.12232766063882602No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAT83260.10163657344365487No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT1457500.0137.178382
ACTGCAA1476000.0136.123951
CCGCAAT196850.0134.438052
ACCGCAA205550.0129.256591
CGCAATT218700.0129.176943
TGCAATT1724400.0128.246413
GCAATTT2447600.0102.811844
CAATTTC2473400.0101.607255
AATTTCC2462550.0101.606976
TTTCCAC2457450.0100.99178
ATTTCCA2497950.099.755937
TTCCACA2492600.099.733299
CGGCAAT26900.098.568712
ACGGCAA34950.076.955091
TATGCAA82950.073.436251
ATGCAAT211250.069.153442
ATTGCAA84650.058.4794161
GGCAATT52600.053.9991343
TCGCAAT13450.046.988922
ACGCAAT27200.046.7377322
Produced by FastQC (version 0.11.5)