FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483812

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483812
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7661997
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT3809604.972071902403512Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAA157490.20554693508754965No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGCAAAA134700.17580273132448368No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAT114510.14945189876738402No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGCATTT97850.12770822019376932No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAGA92240.12038636924551131No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAG84940.11085882701337524No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGCTTTT84270.10998438135645316No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGGAAA82640.1078569986388666No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGCAAAG77220.10078312481719844No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT1945600.0142.011752
ACTGCAA1965500.0141.277241
CCGCAAT266300.0139.50522
ACCGCAA271400.0137.406431
TGCAATT2187900.0134.704683
CGCAATT290100.0132.842153
CGGCAAT33700.0113.623062
GCAATTT3090000.0108.867884
CAATTTC3099300.0108.473215
AATTTCC3098450.0108.13436
TTTCCAC3095600.0107.506158
TTCCACA3105350.0107.4090049
ATTTCCA3126550.0106.87387
ACGGCAA41950.091.860351
ATTGCAA92850.089.661871
GGCAATT44850.086.6705253
TCGCAAT12450.065.946972
GCAATTC53200.061.457964
ATGCAAT171050.058.7893682
AATGCAA120350.056.064261