FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483813

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483813
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7661997
Sequences flagged as poor quality0
Sequence length6
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTG233170.30432014003659885No Hit
GTGGTG168770.22026894555035717No Hit
TGGGGG168730.22021673983949616No Hit
GTGGGT160720.20976254623957696No Hit
GATGGT157650.2057557579309937No Hit
AGGGGG157110.20505098083436996No Hit
GGGGGG152350.19883850124190863No Hit
AGTGCG146280.19091628461874885No Hit
GGGCGG143770.18764037626221988No Hit
GGGGCG141430.18458634217685024No Hit
GTAGGC140870.18385546222479596No Hit
GTGGGG135020.17622037701137183No Hit
TGGGTT134300.17528067421587348No Hit
GGGGGC128610.16785441184589345No Hit
TGCGGG126880.16559651485115434No Hit
GGGTGT126300.16483953204366955No Hit
GTCGGG118210.15428092702202834No Hit
CCGGGG116620.1522057500153028No Hit
GGCGGT115910.1512790986475197No Hit
GGGGGT114470.149399693056523No Hit
GGACGG111940.14609768184456348No Hit
CTGGGG110630.1443879448138651No Hit
GCGGGG110440.14413996768727527No Hit
GAGGGG109850.14336993345207524No Hit
GTGTGG109010.1422736135239938No Hit
TGTGGC108860.142077842108265No Hit
CGCGGG108820.14202563639740395No Hit
GGGAGT107930.14086405933074628No Hit
TTGGCT107800.14069439077044796No Hit
AGGTGG102610.13392069978623067No Hit
CGTCGG101820.1328896369967255No Hit
GGGTGG101060.13189772849036616No Hit
GGTTTC98780.12892200297128803No Hit
GGTGTA98510.12856961442297615No Hit
ATGGTG97470.12721226594058963No Hit
GGGGGA97460.1271992145128744No Hit
GGCTCG97080.1267032602596947No Hit
CGGTGG96560.12602458601850144No Hit
GTGTAG96440.1258679688859184No Hit
GATGGG95780.12500657465671156No Hit
GGTGGG95760.12498047180128104No Hit
AAGTGG94850.12379279187919286No Hit
GTGGCT94760.12367532902975557No Hit
GAAGGG94180.12291834622227078No Hit
GCATCG94140.12286614051140975No Hit
GCGACG93910.12256595767395889No Hit
GTGGCG93860.1225007005353826No Hit
TGGCGG92960.12132607204100966No Hit
GTGCTT92590.12084316921554523No Hit
GTTTGT92100.12020364925749774No Hit
GGGGCA89720.11709740946126708No Hit
AGGGTT88240.11516579815940935No Hit
CATCAA88080.11495697531596527No Hit
GGGGTC87780.11456543248450762No Hit
GCGTGG87610.11434355821334828No Hit
GCCCGG87240.11386065538788386No Hit
GGGGTT85930.11215091835718546No Hit
AGGATG85510.11160275839314476No Hit
ATTTGG85240.11125036984483289No Hit
GGGGAA85040.11098934129052779No Hit
AGCGGG84500.11028456419390402No Hit
TTGGGG84400.11015404991675147No Hit
GGTTGG84380.11012794706132095No Hit
GTTGCG84300.11002353563959894No Hit
GGCGAG84020.10965809566357179No Hit
GGAGGC83950.109566735669565No Hit
TTATGT82770.10802666719916493No Hit
GCGGCG82520.10770038150628354No Hit
TGGTGG81920.10691729584336825No Hit
GTGTGT81770.10672152442763944No Hit
CAGGGG81770.10672152442763944No Hit
GGTCGG81620.1065257530119106No Hit
GGTGGT81460.10631693016846652No Hit
ATCTCG80940.10563825592727327No Hit
TAGGGG80870.1055468959332665No Hit
TGTGGG80710.10533807308982242No Hit
GTGTTG80300.10480296455349694No Hit
AGGCAG79090.10322374179995111No Hit
TTCACT77960.10174893046812729No Hit
GGCGGG77000.10049599340746283No Hit
GTCTGT76960.10044378769660182No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)