FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483814

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483814
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4038580
Sequences flagged as poor quality0
Sequence length151
%GC49

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT53361313.212886707704193Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTC121260.3002540496907329Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAA95830.23728637293306062No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGCAAAA78280.19383050478138356No Hit
AGATCGGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGT69490.172065428937894Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAT65690.16265618113297248No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGCATTT56480.13985113579525477No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAGA53330.1320513645885435No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAG51960.12865908314308494No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGGAAA49000.12132977432661976No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGCTTTT48440.11994314833431553No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGCAAAG44890.11115292999024409No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGCCAGG43540.10781017090165355No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAC43110.10674544022899138No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAGAA42080.10419503885028897No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGGAGG41170.1019417716127946No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCAA1152050.0142.320861
CTGCAAT1166400.0140.051092
CCGCAAT168100.0138.714022
ACCGCAA171850.0135.942281
CGCAATT191850.0130.897193
TGCAATT1434800.0130.681473
CGGCAAT21800.0122.66192
TATGCAA47900.0113.694561
ACGGCAA24150.0111.097061
GCAATTT2077650.0103.288884
CAATTTC2080700.0103.064135
AATTTCC2072500.0103.031286
ATTGCAA65800.0102.9872361
TTTCCAC2067050.0102.497388
TTCCACA2076500.0102.205469
ATTTCCA2084000.0102.1715247
GGCAATT31200.090.596913
ATGCAAT178450.080.054422
CATGCAA44050.079.229831
TCGCAAT8800.075.966742
Produced by FastQC (version 0.11.5)