FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483815

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483815
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4038580
Sequences flagged as poor quality0
Sequence length6
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTGT109220.270441590856192No Hit
GGTGGT107910.2671978764813375No Hit
GGGGGG100510.24887460444017453No Hit
CGGAGT95020.2352807174799063No Hit
GGTTAT94820.23478549391122622No Hit
GTTGGT92280.22849615458898923No Hit
TTGGTT86120.21324326867364274No Hit
GGGGTG83240.20611204928464955No Hit
GCTAAT82050.20316546905100305No Hit
GGTGTG79240.19620757791104795No Hit
TGGGTG78490.19435048952849762No Hit
GGGCTC77190.1911315363320771No Hit
GTGGGG75890.1879125831356566No Hit
GGTCGG75780.18764021017288254No Hit
CGGGGG75470.18687261364142843No Hit
TGGGTC72140.1786271412229051No Hit
TGGTTG70910.1755815162755226No Hit
GTAGAC70670.1749872479931065No Hit
AGGGAC70110.17360062200080226No Hit
GATAGC69900.1730806372536882No Hit
GTAGAA69660.1724863689712721No Hit
AGGTTG69570.17226351836536605No Hit
CGCGTG69540.17218923483006404No Hit
GTATGA68860.17050547469655175No Hit
GTTGGG68640.16996072877100366No Hit
CTGGTG68090.16859886395713344No Hit
AGTGTG67280.1665932085039791No Hit
GGATGG67060.16604846257843103No Hit
GGAGGG66980.16585037315095902No Hit
TGGTGT66980.16585037315095902No Hit
GTTTTG66120.16372091180563464No Hit
CAGGGG66120.16372091180563464No Hit
TGGTGG65290.16166573399561232No Hit
GGGAGG64950.16082385392885618No Hit
GGTAGG64050.15859534786979582No Hit
TTGTAG63330.15681254302254755No Hit
TGGCGG62670.15517830524590326No Hit
GGTGAG62380.15446023107131715No Hit
TTTTGG62270.1541878581085431No Hit
TGGCAC62190.15398976868107106No Hit
TGGAGT62000.153519306290825No Hit
GTCTGT61970.153445022755523No Hit
AGGATC61870.15319741097118295No Hit
GTTTGA61450.15215744147695476No Hit
CATCGG60980.1509936660905566No Hit
GGTCGT60660.15020130838066845No Hit
AATGTG60050.1486908764961942No Hit
GAAGGT59300.14683378811364392No Hit
GGGGGC59180.14653665397243587No Hit
AAGGTA58630.14517478915856563No Hit
GGAGTG58250.14423386437807348No Hit
GCGTTC58100.14386244670156342No Hit
TATGGG57780.14307008899167528No Hit
GGTTGG57700.14287199956420327No Hit
AGGTGT57170.14155965710720106No Hit
TTTTTG57040.14123776178755898No Hit
GTGGAG56850.14076729939731292No Hit
GCTTTG56820.1406930158620109No Hit
GTGGGT56740.14049492643453887No Hit
GGGGTT56540.1399997028658588No Hit
GCGTGT56330.1394797181187447No Hit
TTGGTG55980.13861307687355456No Hit
TACTGG55580.1376226297361944No Hit
CGGTGT55130.13650837670666421No Hit
GGGTTT54550.135072228357492No Hit
TGCCTT54470.13487413893001995No Hit
TGCCGA54030.1337846470789238No Hit
CGCGGG53820.1332646623318097No Hit
GACCTG53590.1326951552278276No Hit
TTTCGC53050.1313580515923914No Hit
GAGAAA52550.1301199926706912No Hit
TAACTG52290.12947620203140708No Hit
CGGTGA52260.12940191849610505No Hit
GGGATT52050.12888193374899098No Hit
TTTGGT51580.12771815836259282No Hit
GCTGTT51410.12729721832921473No Hit
TGGCTT50970.12620772647811854No Hit
CTGAAG50820.12583630880160848No Hit
GGCCAA50680.12548965230353243No Hit
GTTTGG50620.12534108523292842No Hit
AGGGGT50520.12509347344858834No Hit
GTCATA50070.12397922041905818No Hit
GGGATA49820.12336019095820809No Hit
GAGTGC49620.12286496738952798No Hit
GGGGGA49230.12189928143060183No Hit
GATGAG49210.12184975907373384No Hit
AGCTGC49130.1216516696462618No Hit
CGGGAA48770.12076026722263766No Hit
TAGCTA48720.12063646133046765No Hit
GGTACG48710.12061170015203364No Hit
TTGGGT48690.12056217779516562No Hit
TGGGAC48610.1203640883676936No Hit
GGTGTA48500.12009171540491954No Hit
GATTTT48450.11996790951274952No Hit
GTTGAC48390.1198193424421455No Hit
TGGCAT48330.1196707753715415No Hit
GTAATT48320.11964601419310748No Hit
GGTGGG48310.11962125301467348No Hit
ATCGGG48110.11912602944599339No Hit
GGTACT47860.1185069999851433No Hit
GAGAAG47680.11806129877333121No Hit
CTGGGG47440.11746703049091511No Hit
TGGTAC47320.11716989634970709No Hit
GGGGGT47290.11709561281440506No Hit
GAGGTC47120.11667467278102699No Hit
GTATAT46970.11630325510451694No Hit
TTGGAT46970.11630325510451694No Hit
GGTGTT46860.1160308821417429No Hit
TTGTCG46780.11583279271427085No Hit
GAGGGT46690.11560994210836482No Hit
ATGCAC46630.1154613750377608No Hit
ATATCT46590.11536233032402479No Hit
GCTAAG46440.11499091264751471No Hit
GGCGGT46420.11494139029064672No Hit
TTGGGG46060.11404998786702257No Hit
TCGTGG46040.11400046551015455No Hit
GTACAA45830.11348048076304047No Hit
TTAGAT45740.11325763015713444No Hit
TGCTGT45610.11293573483749239No Hit
CGAGTA45490.11263860069628433No Hit
CGCTCG45470.11258907833941634No Hit
TATGAA45030.11149958648832015No Hit
TCTTTC45020.11147482530988616No Hit
TAGGTA44790.11090531820590406No Hit
TGTGGG44490.11016248285288394No Hit
GTGGCT44490.11016248285288394No Hit
GGGCGG44430.11001391578227991No Hit
TTGTGC44420.1099891546038459No Hit
TCCTGG44340.10979106517637388No Hit
GTGAAT44170.10937012514299581No Hit
GGGTTG43970.10887490157431572No Hit
AGCTTA43800.10845396154093764No Hit
AATGAA43580.10790921561538956No Hit
AGTGCG43470.10763684265261551No Hit
GGAAGG43340.10731494733297348No Hit
TTGAAA43300.10721590261923745No Hit
TTTGCG43270.10714161908393545No Hit
TTGTTG42960.10637402255248131No Hit
GCGGCT42760.10587879898380124No Hit
AAAACG42740.10582927662693323No Hit
CGGGGT42680.1056807095563292No Hit
TGAAGG42500.10523500834451714No Hit
TTGCGC42380.10493787420330909No Hit
GTGCCG42270.10466550124053504No Hit
GATGAT42240.10459121770523302No Hit
TCCGGC42130.10431884474245898No Hit
CTAGTG42100.10424456120715697No Hit
CGGTGG42000.10399694942281693No Hit
TGCTTG41970.10392266588751492No Hit
TGTACA41830.10357600938943887No Hit
GTATTG41800.10350172585413685No Hit
TTGGTA41620.10305602464232477No Hit
TCGGGC41590.10298174110702277No Hit
GAGGAA41490.10273412932268274No Hit
CCGCGT41400.10251127871677669No Hit
CTTGGT41360.10241223400304067No Hit
GTTCAG41170.1019417716127946No Hit
GTCTGC41160.10191701043436058No Hit
GCGGGG41130.10184272689905859No Hit
TCAGTG41100.10176844336375658No Hit
TCGGGG41030.10159511511471854No Hit
ATCTGG41010.10154559275785052No Hit
GGAGAA40880.10122369743820847No Hit
CGACCG40820.10107513036760446No Hit
TGTAAC40530.10035705619301835No Hit
CTTTAA40460.10018372794398031No Hit
GGCCGG40420.1000846832302443No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)