FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005483817

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483817
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4598937
Sequences flagged as poor quality0
Sequence length6
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGG296690.6451273413834545No Hit
GGTGGG179740.3908294460219829No Hit
GTGAGG167660.3645625065096565No Hit
GTGGGG166380.3617792546408007No Hit
GAGGGT156890.3411440513318621No Hit
AGGTGG152490.3315766230326704No Hit
GGGCGG148880.32372698299628805No Hit
GGGGTT143660.3123765339686106No Hit
CCGGGG143570.31218083657158163No Hit
GGAGGG138960.30215678101265575No Hit
ATGGGG136910.29769922919144143No Hit
GGGGTG136780.2974165551735108No Hit
GGGGGT134670.2928285384209438No Hit
GGGAGG132480.28806656842657336No Hit
GGGAGT131970.2869576165100761No Hit
GGGGAT119810.2605167237559462No Hit
AGGGGG117800.2561461485556336No Hit
GAGGGG111260.2419254710381986No Hit
AGGGGA111060.2414905879336899No Hit
GGTGGT110640.2405773334142216No Hit
TGGGTG109720.23857687113348153No Hit
GTGACG106740.2320971128763016No Hit
TTATGT105500.2294008376283476No Hit
GGAGAT105250.2288572337477117No Hit
CTGGGG104600.22744386365805835No Hit
GGGGGC104520.22726991041625488No Hit
GGTTGG102800.22352991571747993No Hit
GGGGAC102310.22246445211143356No Hit
CGGGGG100600.21874620156788405No Hit
GGTGAA98310.21376679002125926No Hit
AGAGGG97060.21104877061807978No Hit
GGTGAT97000.2109183056867272No Hit
GGCTGG94640.20578668505352432No Hit
AGGGGT94420.20530831363856475No Hit
AGGACG92770.20172052802636783No Hit
TATGAA90000.19569739702892214No Hit
TGGGGG89240.194044841231789No Hit
CGGGTA88010.1913703101390604No Hit
CACGGT85830.1866300842999154No Hit
ACTGTA85260.18539066745206556No Hit
GATGAC85080.1849992726580077No Hit
AAGGTT84850.1844991570878227No Hit
TGGGCT84550.18384683243105962No Hit
GAGGGC84430.1835859025683544No Hit
GGTACG84160.18299881037726762No Hit
GGTAGA82650.1797154429382268No Hit
AGCGGG82610.17962846631732507No Hit
TGAGGG82110.1785412585560533No Hit
TGAGCG81970.1782368403828972No Hit
GGGGCT80590.17523614696178705No Hit
AGCTGG79990.1739314976482609No Hit
TAGAGG79990.1739314976482609No Hit
GTGGTG79510.17288777819743997No Hit
CGGCGG79300.1724311509377058No Hit
GGCGCG78470.17062638605399466No Hit
CTACGG77890.1693652250509194No Hit
ATTTTA77600.16873464454938172No Hit
AGCGAG77130.16771266925378625No Hit
CACGGC76980.16738650692540472No Hit
CTGGCG76920.16725604199405208No Hit
GGTCGG76650.16666894980296534No Hit
GGAGAC75930.16510337062673397No Hit
GACGGG75890.16501639400583223No Hit
CCGCTG75790.16479895245357787No Hit
GTGTGT75570.16432058103861827No Hit
ATTAGA75090.16327686158779736No Hit
GGCGGG74880.1628202343280632No Hit
GCGGGT74620.16225488629220186No Hit
GGTGAC74490.16197221227427122No Hit
TGGATT74240.1614286083936353No Hit
GGGGCC74090.16110244606525378No Hit
CGGCCG73790.16045012140849071No Hit
CGGTGG73630.16010221492488375No Hit
GCGCGG73160.15908023962928824No Hit
ATGACG72780.15825396173072168No Hit
GGGTAG72780.15825396173072168No Hit
AGGGAG72700.1580800084889182No Hit
AATTGT72650.15797128771279104No Hit
GTGCAG72270.15714500981422447No Hit
GAGAGC71660.15581861634547287No Hit
GGCGGT71430.15531850077528786No Hit
GCAGGG71190.1547966410498774No Hit
GCCGTT71150.15470966442897566No Hit
GGAAGT70910.1541878047035652No Hit
AAGGGG70080.15238303981985402No Hit
GGACAG69880.1519481567153453No Hit
GCGAGG69820.15181769178399268No Hit
GGGGCG69600.1513393203690331No Hit
GCGGAG69480.15107839050632788No Hit
GGGCAG68790.1495780437957728No Hit
AAGTTG68660.14929536977784214No Hit
GGAAAG68360.14864304512107906No Hit
ATGGCG68030.1479254879986397No Hit
AGCACG67930.14770804644638533No Hit
GTAGGC67910.14766455813593446No Hit
GAGCCT67640.1470774659448477No Hit
GGAATG67020.14572932832087065No Hit
GAGGGA66790.14522921275068565No Hit
CGTAAT66240.14403328421328668No Hit
GGGTCG66220.1439897959028358No Hit
TTGAGC66190.1439245634371595No Hit
GGAGGC66130.1437940985058069No Hit
TGTCGT65400.14220677517435007No Hit
GGGCTG65380.1421632868638992No Hit
TAAGGG65380.1421632868638992No Hit
GGGGTC64870.141054334947402No Hit
GTTTTG64800.14090212586082393No Hit
CGATGG64660.14059770768766783No Hit
GTGAGA64640.14055421937721696No Hit
TGAAAG64530.14031503366973716No Hit
TGATTT64260.1397279414786504No Hit
TCGATC64040.1392495700636908No Hit
GGGTGT63940.13903212851143645No Hit
TAGAGC63890.13892340773530928No Hit
GGGCCG63870.13887991942485842No Hit
TTCGGG63850.13883643111440752No Hit
TGGTCA63790.13870596618305492No Hit
TAGTGG63750.13861898956215318No Hit
GAGGTA63460.13798840906061552No Hit
AGGAGG63150.13731434024862704No Hit
TGGCCC62760.136466318194835No Hit
GTCGCA62620.1361619000216789No Hit
TGCGTT62490.13587922600374827No Hit
GGGAGA62000.1348137623977019No Hit
CGGCCC60970.13257411440948202No Hit
GGGACG60810.13222620792587506No Hit
GGGTAT60500.13155213911388652No Hit
GAGCGG59420.12920377034953948No Hit
GGGGTA59350.1290515612629614No Hit
TTAGGT59330.12900807295251054No Hit
AGAGGT58990.12826877167484574No Hit
CTGTGT58650.1275294703971809No Hit
TCGGGC58650.1275294703971809No Hit
GCGGCA58590.1273990054658283No Hit
TAGTGT58530.12726854053447567No Hit
TGGGGT58320.12681191327474153No Hit
CGTGGG58280.1267249366538398No Hit
CAGGGA58170.12648575094636No Hit
AGGCAG58130.12639877432545826No Hit
CGGGGA57990.12609435615230216No Hit
GTGCAT57960.12602912368662583No Hit
GTACAG57630.12531156656418646No Hit
AGAAGT57460.12494191592535406No Hit
TGGATG57000.12394168478498402No Hit
CTGGAG56840.12359377830137705No Hit
CGCCGG56790.12348505752524987No Hit
TTTGCT56740.12337633674912268No Hit
GGTATG56260.12233261729830175No Hit
GTGCGG56240.12228912898785088No Hit
TTTACG56040.12185424588334218No Hit
TGTCGA55900.12154982771018608No Hit
ATAGGG55770.1212671536922554No Hit
TTTGAT55360.12037564332801254No Hit
ACGGAG55060.11972331867124947No Hit
TGCGGG54760.1190709940144864No Hit
TGGAGT54310.11809250702934178No Hit
TAGCGA54180.11780983301141112No Hit
AGTAGG54120.1176793680800585No Hit
CTTGCG54060.11754890314870589No Hit
TGTAGT54050.11752715899348044No Hit
GGGGAA54040.11750541483825502No Hit
AGGTTG54030.11748367068302958No Hit
AAGCCT53830.11704878757852087No Hit
TGGGCG53680.11672262525013932No Hit
TGGGAG53620.11659216031878672No Hit
AGCCGG53530.1163964629217578No Hit
GGTAGT53500.11633123045608149No Hit
CGGAGT53440.11620076552472886No Hit
CGGGAC53410.11613553305905257No Hit
TGTAGG52650.11448297726191943No Hit
AGAAGG52620.11441774479624314No Hit
GGAAGG52530.11422204739921422No Hit
CTTAGT52530.11422204739921422No Hit
GTTCGA52510.11417855908876333No Hit
AATCGG52370.11387414091560723No Hit
CCGCAC52250.11361321105290202No Hit
GCGCCG52030.11313483963794242No Hit
GGGTGA50970.11082995918404624No Hit
CGCGGG50890.11065600594224274No Hit
GGTAAG50850.11056902932134101No Hit
GTGTGG50590.11000368128547967No Hit
GGGGGA50460.10972100726754899No Hit
GCGGGG50330.10943833324961834No Hit
AGGCTG50000.10872077612717895No Hit
TGTGTA49830.10835112548834654No Hit
AGGAAG49720.10811193978086675No Hit
TTAAAA49580.10780752160771065No Hit
CACAAA49560.10776403329725978No Hit
GGTTCG49350.10730740603752563No Hit
GTTCGT49340.10728566188230021No Hit
GTATTT49290.10717694110617303No Hit
GGGTGG49110.10678554631211516No Hit
GTACCG49100.10676380215688974No Hit
GTGGTC49060.106676825535988No Hit
GCCGGC49060.106676825535988No Hit
CATAGG49000.10654636060463538No Hit
CGGTCA48940.10641589567328277No Hit
GAGAAA48930.10639415151805733No Hit
CAGAAC48890.10630717489715559No Hit
CGCTGG48870.10626368658670472No Hit
GTCAAG48860.10624194243147927No Hit
GTTGGT48500.1054591528433636No Hit
GGCCGC48400.10524171129110924No Hit
CGAGTT48270.10495903727317855No Hit
ATGTAG47980.10432845677164093No Hit
GGCTGT47970.10430671261641548No Hit
GGTCAA47960.10428496846119006No Hit
TGGGCA47940.1042414801507392No Hit
GCTAAT47920.10419799184028831No Hit
CTGGTT47910.10417624768506288No Hit
TTACGT47860.1040675269089357No Hit
TCGAGT47820.10398055028803396No Hit
CGGACT47710.10374136458055416No Hit
AGTGCG47440.1031542723894674No Hit
GGATTG47340.10293683083721303No Hit
TGACCT47050.1023062503356754No Hit
AAGGGC46930.10204532047297017No Hit
AAACAA46820.10180613476549039No Hit
CTTGGG46720.10158869321323602No Hit
GCGGCT46710.10156694905801057No Hit
CCGAGG46480.10106683348782555No Hit
GAGTCG46470.10104508933260012No Hit
CTTATG46420.10093636855647295No Hit
GGGGAG46380.1008493919355712No Hit
TTTGGC46280.10063195038331685No Hit
GATGTG46230.10052322960718967No Hit
GGCAGG46120.10028404389970988No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)