FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483818

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483818
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences997381
Sequences flagged as poor quality0
Sequence length151
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT26131226.199817321565178Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTC64350.6451897519603842Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGT53020.5315922400767611Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCG19200.19250416841708434Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTA18500.18548578727687814Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAA16980.17024587394385896No Hit
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT14840.14878968017237143Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC14480.1451802270145511RNA PCR Primer, Index 16 (96% over 25bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGCAAAA14430.14467891407596495No Hit
AGATCGGAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC14100.14137024868129633RNA PCR Primer, Index 16 (96% over 25bp)
GTATTAGAAGACAACAGTTTAGCAATAGTCAAGGAAAATGGAGCCATTGT12090.1212174685501328No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAT11880.11911195420807094No Hit
AGATCGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC10920.10948674578721673Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
CTCTTCCGATCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTC10670.10698018109428593Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCAA216700.0138.835861
CTGCAAT217550.0137.667482
CCGCAAT31200.0134.803472
CGCAATT33550.0130.774033
TGCAATT260400.0126.813523
ACCGCAA34450.0123.4629751
GCAATTT389750.097.0275654
CAATTTC389850.096.853625
AATTTCC389450.096.5717246
TTTCCAC385300.096.459568
ATTTCCA389500.096.1823357
TTCCACA390200.095.433999
GGCAATT5050.093.49733
ATTGCAA10900.086.713531
CGGCAAT5100.085.4590762
TGTATCG702.910383E-1183.092541
TATGCAA11700.076.434481
GTTCGCG607.924791E-872.640212
CGTAGCG801.0004442E-1072.476347
TTAGCGG1300.072.476338
Produced by FastQC (version 0.11.5)