FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483819

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483819
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences997381
Sequences flagged as poor quality0
Sequence length6
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGGTG28670.2874528389853025No Hit
AGGGAG28640.2871520512221508No Hit
ATCCGG26700.26770110920500795No Hit
TCAGGG25860.2592790518367605No Hit
CGGGCC25270.2533635591614438No Hit
GTGGGA24690.2475483290738444No Hit
GGGTGG23880.23942705946874862No Hit
GCGGGG23280.23341130420571476No Hit
GGCTTG22730.22789686188126707No Hit
GTGGGG22700.22759607411811533No Hit
GGATGG22500.22559082236377073No Hit
TGATAG22190.22248268214453654No Hit
GGGGGG21950.22007638003932298No Hit
GGGGAC21930.2198758548638885No Hit
AAGCAG21440.21496298806574415No Hit
AGGAGG21270.21325852407455126No Hit
GGGGCG20150.20202911425022133No Hit
ATCTCG19970.20022438767131118No Hit
TGGTTG19850.19902123661870436No Hit
GGGACG19710.19761756039066314No Hit
GTGGTT19580.19631414675033912No Hit
TGGCGG19510.1956123086363185No Hit
GGGAGG18810.1885939274961123No Hit
GCTGGG18720.18769156420665722No Hit
GGGGGT18210.18257817223307843No Hit
GGCGGG17950.17997134495243042No Hit
GTCGGC17580.17626162920689287No Hit
TGTGCT17350.17395558968939653No Hit
GTTTGT16850.16894246030353496No Hit
GGGCGC16840.16884219771581774No Hit
ATTAGG16650.16693720854919034No Hit
GCTCGG16630.16673668337375586No Hit
TGGGGT16630.16673668337375586No Hit
TGGGTG16610.1665361581983214No Hit
TAAGAT16570.16613510784745247No Hit
GAGCGT16380.16423011868082507No Hit
GAGAGG16350.1639293309176734No Hit
GATGCG16070.1611219784615909No Hit
ATGGGG16010.16052040293528752No Hit
ATTTAG15960.16001908999670136No Hit
TGCGGC15950.1599188274089841No Hit
GGTGGA15930.15971830223354966No Hit
ACGAGG15880.1592169892949635No Hit
GGGTTG15600.156409636838881No Hit
AGAGGG15100.15139650745301947No Hit
ATTAGT15040.15079493192671606No Hit
GCTGCG15000.15039388157584715No Hit
GGGATA14890.1492909931109576No Hit
AGGGCG14780.14818810464606805No Hit
GCCCGG14740.14778705429519914No Hit
GTTTGG14730.1476867917074819No Hit
TTGTGG14620.14658390324259235No Hit
GTCGGG14590.14628311547944067No Hit
TAGAAG14520.14558127736542004No Hit
CTTGTT14510.1454810147777028No Hit
AGGGAT14510.1454810147777028No Hit
GGGCCG14500.14538075218998556No Hit
GGAAAC14460.14497970183911665No Hit
CGGGAC14460.14497970183911665No Hit
CACATC14350.14387681337422709No Hit
TAGCGG14320.1435760256110754No Hit
GAGCTG14170.14207208679531694No Hit
GGTCGC14050.14086893574271017No Hit
GTGGAG13890.13926473433923447No Hit
CCTGGG13870.1390642091638No Hit
CGGGGG13670.13705895740945537No Hit
AAGAAG13520.1355550185936969No Hit
GGTAGT13500.13535449341826242No Hit
ACTGCG13450.13485318047967626No Hit
GCGTTG13430.1346526553042418No Hit
GAAGTC13410.13445213012880736No Hit
CGGGGC13280.13314871648848334No Hit
GTGTGT13260.13294819131304889No Hit
CTAGGC13240.1327476661376144No Hit
GTCGAA13230.13264740354989718No Hit
AGGACA13150.13184530284815932No Hit
CTTTTA13140.1317450402604421No Hit
AGAGTG13130.13164477767272487No Hit
AAGAAT13060.13094293955870426No Hit
TGCGAC12910.1294390007429458No Hit
GGGGCA12860.12893768780435963No Hit
CTCCGA12830.12863690004120792No Hit
GTGGTG12800.12833611227805622No Hit
GTGCGG12760.1279350619271873No Hit
GTGGTC12720.12753401157631838No Hit
CACATT12690.12723322381316668No Hit
ACCAAC12660.126932436050015No Hit
GTGGCG12580.12613033534827714No Hit
CCTTAG12560.12592981017284266No Hit
CGTGTG12520.12552875982197376No Hit
TGTGGT12510.12542849723425653No Hit
TGGGTC12510.12542849723425653No Hit
GAATGT12490.12522797205882205No Hit
GTGGAT12480.12512770947110483No Hit
AGAGGA12460.12492718429567035No Hit
GACGTG12440.1247266591202359No Hit
GGTAGG12330.12362377065534635No Hit
ATGAGA12300.12332298289219466No Hit
GGGGTT12280.12312245771676018No Hit
GTTGGG12270.12302219512904296No Hit
GTAGGT12260.12292193254132572No Hit
AGGGGT12170.12201956925187066No Hit
GGGTGA12160.12191930666415342No Hit
TAGGGT12140.12171878148871897No Hit
GAATAG12110.12141799372556725No Hit
TGCCAC12090.1212174685501328No Hit
GATCGT12010.12041536784839495No Hit
GGCGGA12000.12031510526067772No Hit
GATGGC11990.12021484267296048No Hit
ATCGCG11890.11921221679578817No Hit
TCGTGG11860.11891142903263648No Hit
GCATTA11850.11881116644491925No Hit
GGGGTG11830.11861064126948478No Hit
GGGGAT11830.11861064126948478No Hit
CGTGAG11830.11861064126948478No Hit
ACGGCG11800.11830985350633309No Hit
GGGATG11790.11820959091861587No Hit
TGAGGA11790.11820959091861587No Hit
TGATAT11780.11810932833089863No Hit
AACGAT11750.11780854056774692No Hit
GCGCTG11730.11760801539231246No Hit
GTCTGA11710.117407490216878No Hit
AGTCCG11660.11690617727829185No Hit
ATATGA11500.11530197587481614No Hit
GCAGAT11470.11500118811166446No Hit
GACTGA11450.11480066293623No Hit
GTGTCT11350.11379803705905767No Hit
GTGGGT11290.11319646153275428No Hit
GGGGTA11260.11289567376960259No Hit
GTCCGG11230.11259488600645089No Hit
TTGTCT11110.11139173495384412No Hit
GGCTGG11080.11109094719069243No Hit
TGTCGG11030.11058963425210626No Hit
TTGCAG11010.1103891090766718No Hit
GCGATT11010.1103891090766718No Hit
CTAGAT10980.11008832131352012No Hit
TGTGTG10970.10998805872580288No Hit
ATTTAT10970.10998805872580288No Hit
CTGCAG10960.10988779613808565No Hit
GACATT10950.10978753355036842No Hit
AGTTGG10900.10928622061178227No Hit
CTGGTT10850.1087849076731961No Hit
TGTTAC10800.10828359473460994No Hit
TGGTTT10760.10788254438374102No Hit
GGGTTC10750.1077822817960238No Hit
ATGCGG10740.10768201920830656No Hit
GTGACT10730.10758175662058933No Hit
TATGGG10680.10708044368200316No Hit
ATGTAA10630.10657913074341702No Hit
CGGGTT10540.10567676745396191No Hit
ATGCGC10530.10557650486624469No Hit
TACGTG10480.10507519192765853No Hit
ACGGGG10470.1049749293399413No Hit
TACGAT10420.10447361640135515No Hit
CATGGA10410.10437335381363792No Hit
GGGCGT10380.10407256605048623No Hit
GTAGAG10290.10317020276103114No Hit
TGCGTA10280.1030699401733139No Hit
AGCTTT10250.10276915241016221No Hit
GGGTGC10240.10266888982244499No Hit
CTCAGT10230.10256862723472776No Hit
AGGCAG10200.10226783947157607No Hit
TGTATA10150.1017665265329899No Hit
GTCTGG10150.1017665265329899No Hit
TGGAGT10130.10156600135755543No Hit
GCCATA10120.1014657387698382No Hit
GATGAA10080.10106468841896929No Hit
AGGGGG10030.10056337548038313No Hit
TTGGTA10020.10046311289266588No Hit
CGTACA10000.10026258771723143No Hit
ACTGGC9990.10016232512951419No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)