FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483822

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483822
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences666034
Sequences flagged as poor quality0
Sequence length151
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT20499230.77800832990508Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTC33750.5067308876123442Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGT30660.4603368596798362Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCG15500.23272085208863208Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAA15070.2262647252242378No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGCAAAA12270.18422482936306556No Hit
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT11740.1762672776464865Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC11290.16951086581165525RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGGAAAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC11090.16650801610728583RNA PCR Primer, Index 16 (96% over 25bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAT10370.15569775717155582No Hit
AGATCGGAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTA9680.1453379256914812Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGCATTT9240.13873165634186843No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAG8580.12882225231744926No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAGA8500.12762111243570148No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGCAAAG8110.12176555551218105No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGCCAGG7690.11545957113300521No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGCTTTT7660.1150091436773498No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGGAAA7630.11455871622169439No Hit
AGATCGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC7610.11425843125125745Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAC7520.11290714888429118No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGCAAAT6920.10389859977118286No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAATT6710.10074560758159493No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCAA182200.0142.08881
CTGCAAT182800.0141.009692
CCGCAAT27000.0135.653412
TGCAATT216000.0132.659093
CGCAATT30400.0126.457933
ACCGCAA30300.0121.441231
GGCGTTA458.54925E-11112.747478
GCAATTT315750.0103.769124
CAATTTC316850.0103.408875
TTTCCAC314000.0102.9269568
AATTTCC317000.0102.867466
ATTTCCA316450.0102.5881657
TTCCACA315850.0102.392939
GGCAATT3700.0102.132893
CGGCAAT3650.0101.5409552
TATGCAA4550.099.0919951
ATTGCAA7150.092.553671
GCGTTAA554.1654857E-1092.2479259
GCGGACT554.1654857E-1092.2479257
ATGCAAT27600.087.416412
Produced by FastQC (version 0.11.5)