FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005483823

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483823
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences666034
Sequences flagged as poor quality0
Sequence length6
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGG27340.41048955458730335No Hit
GGAGGG24510.3679992312704757No Hit
AAAGTG23040.3459282859433603No Hit
GGGGTG22690.3406732989607137No Hit
GGGGGA22300.3348177420371933No Hit
GGGGGC22160.33271574724413466No Hit
GCTTTG22010.33046360996585755No Hit
CGGAGG21870.328361615172799No Hit
GGCACG21490.322656200734497No Hit
GGTTGG19540.2933784161168949No Hit
GTTACG18810.28241801469594646No Hit
TGGTCG18660.28016587741766935No Hit
GTGAGG18110.2719080407306534No Hit
CATAGG18060.2711573283045611No Hit
AGGGGG17840.26785419362975466No Hit
GGGGTT17650.26500148641060367No Hit
CGCTGT17620.26455105895494824No Hit
GGGATC16870.25329037256356285No Hit
GGGCCT16740.2513385202557227No Hit
GAACGT16630.24968695291831947No Hit
CGGGGT16590.2490863829774456No Hit
GGGTAG16270.2442818234504545No Hit
AGCTTG16120.2420296861721774No Hit
GTCGTA15640.23482284688169072No Hit
CGGTAG15510.23287099457385058No Hit
ATGGTG15330.2301684298399181No Hit
CAAGTG15150.2274658651059856No Hit
ATGTCG15060.22611458273901933No Hit
GCGGTG15050.22596444025380086No Hit
TGACCG14790.22206073563812057No Hit
TAAGTG14520.2180068885372218No Hit
GGTGGT14480.21740631859634793No Hit
GGTGAA14460.21710603362591097No Hit
AAGTTG14390.21605503622938166No Hit
TGAAAA14380.2159048937441632No Hit
GGGACT14340.21530432380328932No Hit
GGAACT14310.21485389634763394No Hit
AATTAG14230.21365275646588613No Hit
GGACAG14110.21185104664326448No Hit
CATATG14060.2111003342171721No Hit
GGGGTA14000.21019947930586125No Hit
CTGCAG13860.20809748451280263No Hit
GTTTGT13820.20749691457192876No Hit
GTGGGG13730.2061456322049625No Hit
GGGTGT13570.20374335244146696No Hit
GGTGGG13540.20329292498581153No Hit
GTATGT13460.20209178510406373No Hit
TAGCCG13330.2001399327962236No Hit
ACTCGG13060.19608608569532487No Hit
TGCGAA13020.19548551575445097No Hit
GGTTAA12910.1938339484170478No Hit
CTGCTG12870.1932333784761739No Hit
GTGTCA12840.19278295102051848No Hit
TCGGGC12830.1926328085353No Hit
GTTTCC12810.19233252356486305No Hit
CGCAGT12750.1914316686535522No Hit
TGTAGG12710.19083109871267834No Hit
GGGCGT12660.19008038628658597No Hit
CCGGCC12650.1899302438013675No Hit
ATGGGC12580.1888792464048382No Hit
TGTTAG12500.18767810652309042No Hit
TGACAC12450.18692739409699807No Hit
GGGTGG12320.18497554178915793No Hit
GGAAAC12170.18272340451088082No Hit
TGGGGG12170.18272340451088082No Hit
TCGAGG12160.18257326202566235No Hit
TCGGGG12110.18182254959957No Hit
TGGCAT12060.18107183717347763No Hit
CAGGGG12030.1806214097178222No Hit
ATGTCT11870.17821912995432665No Hit
TGTGTG11860.17806898746910818No Hit
GGGTTC11850.1779188449838897No Hit
GGGGCG11750.176417420131705No Hit
AGGGTT11750.176417420131705No Hit
CCGAAT11700.17566670770561263No Hit
TGCATG11570.17371485539777248No Hit
CGGGTC11510.17281400048646164No Hit
CTAATT11500.17266385800124318No Hit
GTGGGT11480.17236357303080624No Hit
CTGTGC11450.1719131455751508No Hit
AGGGGT11340.17026157823774762No Hit
TTGTTC11330.17011143575252916No Hit
CATCGA11250.16891029587078138No Hit
TCTGTG11180.16785929847425207No Hit
AATGTC11160.16755901350381514No Hit
GGGGAG11110.16680830107772276No Hit
GGGATA11080.16635787362206733No Hit
GCTGTT11050.16590744616641193No Hit
TAAGGT11050.16590744616641193No Hit
CTAGTA11030.165607161195975No Hit
GCCCAA11020.16545701871075652No Hit
GGGAAA11000.16515673374031958No Hit
CGGCGA10990.1650065912551011No Hit
CAGTAG10970.16470630628466415No Hit
TGTGAC10960.16455616379944568No Hit
CGGGTG10850.16290459646204247No Hit
TGTGGG10790.16200374155073163No Hit
GGGGCT10770.1617034565802947No Hit
GATTAA10640.15975160427245458No Hit
ATGAAA10600.15915103433158068No Hit
TGGATA10570.15870060687592527No Hit
GGCTTG10550.1584003219054883No Hit
CGCTCG10510.15779975196461443No Hit
GATGTC10380.15584789965677426No Hit
ATTAGT10300.1546467597750265No Hit
CCATTA10290.154496617289808No Hit
GTTCGT10120.151944195041094No Hit
GACGTT10100.15164391007065706No Hit
TGCAAG10080.15134362510022012No Hit
ATCGTG9970.1496920577628169No Hit
TGCGGG9950.14939177279237997No Hit
TGCTCG9910.14879120285150607No Hit
GCCGCT9880.14834077539585067No Hit
GTTTAC9840.14774020545497676No Hit
AGACGG9790.1469894930288844No Hit
AGCAGC9760.146539065573229No Hit
AGAATG9710.14578835314713665No Hit
ACGTGC9700.14563821066191815No Hit
CGCGGG9670.14518778320626274No Hit
ATATTG9670.14518778320626274No Hit
TCTTTG9650.1448874982358258No Hit
CGTGTG9530.14308578841320413No Hit
GACGTG9530.14308578841320413No Hit
ATGTGC9500.1426353609575487No Hit
AGTTGC9480.14233507598711176No Hit
ACGCTT9440.14173450604623788No Hit
GCATAT9430.1415843635610194No Hit
CGCGAC9400.14113393610536398No Hit
CGAATG9360.14053336616449008No Hit
GACGAT9280.13933222628274233No Hit
GAGCAT9250.1388817988270869No Hit
GGTCTA9210.13828122888621303No Hit
GCTGGG9200.13813108640099453No Hit
TGCGAC9200.13813108640099453No Hit
GGGACA9180.1378308014305576No Hit
GACTAG9170.13768065894533912No Hit
TCAGGC9160.13753051646012066No Hit
GAGTTT9150.1373803739749022No Hit
GAGGTC9130.13708008900446522No Hit
GGTCGG9120.13692994651924678No Hit
GCTGAA9100.13662966154880982No Hit
TAGGTT9100.13662966154880982No Hit
TGGAGT9070.1361792340931544No Hit
GAGTAC9070.1361792340931544No Hit
GGCTTA9050.13587894912271747No Hit
ACATCT9000.13512823669662508No Hit
CGAGCT9000.13512823669662508No Hit
AGCGGC8970.13467780924096967No Hit
TGGTGG8890.1334766693592219No Hit
TGAGAC8880.13332652687400343No Hit
GGGAAG8860.1330262419035665No Hit
CGGGGG8820.1324256719626926No Hit
GTCTGG8780.1318251020218187No Hit
GCGTTT8690.13047381965485247No Hit
GTTGTT8680.13032367716963397No Hit
ATGGTT8610.12927267977310467No Hit
CCCAGT8580.12882225231744926No Hit
CACGGC8540.12822168237657536No Hit
ACGACG8530.12807153989135692No Hit
GAGGGT8510.12777125492091995No Hit
CGTATG8500.12762111243570148No Hit
GGATAG8500.12762111243570148No Hit
ATGTGT8500.12762111243570148No Hit
TTGGAC8500.12762111243570148No Hit
TATCGG8480.12732082746526452No Hit
GTTTTT8450.1268704000096091No Hit
GAGCTT8430.12657011503917218No Hit
TTCTGG8400.12611968758351677No Hit
GCTTGC8380.1258194026130798No Hit
CGAGTT8350.1253689751574244No Hit
GTCAGG8310.1247684052165505No Hit
ATTGTC8280.12431797776089508No Hit
GGCACA8280.12431797776089508No Hit
GTAAGA8260.12401769279045814No Hit
ATAGGT8240.1237174078200212No Hit
TCTCAG8240.1237174078200212No Hit
TGACCC8230.12356726533480274No Hit
GCTCTG8180.12281655290871035No Hit
ATCTCC8150.12236612545305495No Hit
GAGTGC8110.12176555551218105No Hit
ATTGGG8080.12131512805652564No Hit
GAAGGG8040.12071455811565177No Hit
TAAATG8000.12011398817477786No Hit
GCATCC7870.11816213586693773No Hit
GTAAAG7840.11771170841128231No Hit
TAGAAT7830.11756156592606383No Hit
TGTGGA7810.11726128095562689No Hit
TTGAGG7810.11726128095562689No Hit
GCGTGT7740.11621028355909759No Hit
TTAGTC7720.11590999858866063No Hit
ACAAGG7700.1156097136182237No Hit
GAAGCT7700.1156097136182237No Hit
AGTACA7690.11545957113300521No Hit
ACATGT7620.11440857373647591No Hit
GCACGC7580.11380800379560203No Hit
TGATGT7560.11350771882516508No Hit
AGAGCC7530.11305729136950966No Hit
GGGTCG7520.11290714888429118No Hit
CGGTTG7510.11275700639907273No Hit
ACACGG7480.1123065789434173No Hit
GCTTAC7480.1123065789434173No Hit
ACTTAA7480.1123065789434173No Hit
TCGCGT7460.11200629397298036No Hit
AGATTG7410.111255581546888No Hit
AGGCGA7390.11095529657645105No Hit
GTGTGG7350.11035472663557717No Hit
AGTATT7330.11005444166514021No Hit
TGTTGG7320.10990429917992174No Hit
CGAACT7230.10855301681295548No Hit
AGTTAG7190.1079524468720816No Hit
ATGTTT7170.10765216190164466No Hit
GGGGGT7120.1069014494755523No Hit
GGTATT7070.10615073704945993No Hit
ATAATA7060.10600059456424146No Hit
AAACGG7040.10570030959380453No Hit
AACTAA7010.1052498821381491No Hit
TTAGGT6950.10434902722683828No Hit
CAGTGC6940.1041988847416198No Hit
TTGATC6940.1041988847416198No Hit
GTGGTA6920.10389859977118286No Hit
TGTGAG6880.10329802983030896No Hit
TTTGGA6880.10329802983030896No Hit
GGGGTC6870.10314788734509049No Hit
GCCTGG6870.10314788734509049No Hit
CGACGG6850.10284760237465355No Hit
GTGTGA6830.10254731740421659No Hit
GCGGTA6830.10254731740421659No Hit
TGTTTC6820.10239717491899814No Hit
TCTTGT6810.10224703243377965No Hit
GTGGTG6730.10104589255203186No Hit
GTGGAG6720.10089575006681341No Hit
TCGGGT6710.10074560758159493No Hit
ATGATA6680.10029518012593952No Hit
CAGTGT6670.10014503764072104No Hit
GGGAGG6670.10014503764072104No Hit
TGCGTG6670.10014503764072104No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)