FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483824

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483824
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6117758
Sequences flagged as poor quality0
Sequence length151
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT102431516.74330694349139Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT342570.5599600376477788Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT209140.34185726208849715Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT195460.3194961291375043Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT176590.28865149618536723Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTC70860.11582674568036198Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC69650.11384889693250372RNA PCR Primer, Index 16 (96% over 25bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT68720.1123287321924143Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC68510.11198546918658764RNA PCR Primer, Index 16 (96% over 25bp)
AGGTCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT67530.11038357515939663Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT564250.0134.186612
ACTGCAA576150.0131.569631
TGCAATT632600.0128.822723
CGCAATT73800.0119.385793
CCGCAAT71100.0118.811782
GCAATTT818350.0111.975744
AATTTCC877400.0110.795916
CAATTTC851450.0110.254525
ATTTCCA897550.0107.996457
ACCGCAA78450.0107.744811
TTTCCAC896650.0107.708728
TTCCACA933650.0103.6024869
GACCGGA39750.095.549912
AGACCGG41250.091.424561
AGATTGG69850.085.780881
GATTGGA70350.084.499642
ATTGGAA74950.079.210613
ACCGGAA48200.077.887453
ACGGAAG42900.075.638414
CGGCAAT19700.073.49942
Produced by FastQC (version 0.11.5)