FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483830

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483830
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3988335
Sequences flagged as poor quality0
Sequence length151
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT2134735.352434035756776Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAA77870.19524438142733747No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGCAAAA65310.16375254335455774No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAT54680.13709981734232457No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGCATTT48410.12137897142541938No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAG44460.11147508922896396No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAGA44130.11064767628596896No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGGAAA40000.10029247793878901No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCAA926950.0141.276441
CTGCAAT936500.0139.30852
CCGCAAT123400.0137.089922
ACCGCAA124200.0136.790511
TGCAATT1115450.0129.29443
CGCAATT140300.0128.42923
CGGCAAT15350.0120.458582
GCAATTT1533050.0106.537754
AATTTCC1544650.0106.362076
CAATTTC1538900.0106.272775
TTTCCAC1544700.0105.5272458
ATTTCCA1555850.0105.274247
TTCCACA1558000.0104.8112269
ATTGCAA48900.094.431
ACGGCAA21050.088.642661
GGCAATT21650.088.418083
TATGCAA26850.087.882181
ATGCAAT115250.068.3903052
CATGCAA28400.067.491231
ACGCAAT13300.064.878572