FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483832

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483832
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10020122
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT3726303.718816996439764Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT264430.26389898246747895Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT134760.13448938046862105Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT123960.12371106858778765Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT111350.11112639147507386Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT738300.0126.8993152
ACTGCAA764950.0122.941761
TGCAATT844800.0122.185793
CGCAATT101900.0117.389973
ACCGCAA96750.0115.555441
CCGCAAT97850.0114.2232362
GCAATTT1071950.0109.0000154
AATTTCC1189500.0107.098546
CAATTTC1135150.0106.2555545
TTTCCAC1229050.0103.012328
ATTTCCA1238750.0102.480727
TTCCACA1288800.098.377189
CGGCAAT29400.069.749842
GACCGGA34350.065.837762
AGACCGG38200.059.0290571
ACGGCAA36150.056.742481
GTTCCAC82850.051.5208055
ACCGGAA45600.049.5946433
GATTGGA95550.049.3918652
AGATTGG99100.047.636361
Produced by FastQC (version 0.11.5)