FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483834

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483834
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8938206
Sequences flagged as poor quality0
Sequence length151
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT92680110.368982321508366Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT236550.264650423138603Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT210530.23553943598972768Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT153120.17130954466701706Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT146580.1639926401338255Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT563450.0127.316962
TGCAATT621750.0125.239553
ACTGCAA577950.0124.416161
CGCAATT71950.0112.6416553
GCAATTT789650.0110.2968444
CCGCAAT70800.0107.1627352
AATTTCC857100.0106.9657066
CAATTTC835100.0106.9172445
TTTCCAC878150.0103.888588
ATTTCCA896150.0102.069497
ACCGCAA74550.0101.848071
TTCCACA911200.0100.3040549
GACCGGA43400.077.88282
AGACCGG44750.074.778491
CGGCAAT22400.059.582012
ACCGGAA58200.058.4691163
GATTGGA80850.055.5336152
AGATTGG81000.055.2032281
ATTGGAA88950.050.0024343
ACGGAAG49700.047.299924
Produced by FastQC (version 0.11.5)