FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483837

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483837
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences464477
Sequences flagged as poor quality0
Sequence length6
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGCG14700.3164849927983517No Hit
GGGGGG11360.2445761576999507No Hit
GGTGGG10750.23144310697838644No Hit
ACGGAT10400.2239077500069971No Hit
TGGAGG10080.2170182807760126No Hit
GTTCTG8680.18687685289045528No Hit
GGCCTG8610.18536978149617742No Hit
GTTGGT8520.18343211827496303No Hit
GGTGGA8320.1791262000055977No Hit
GGGTTT8160.17568146539010543No Hit
GGCGGG8140.17525087356316887No Hit
AGGTCA8110.1746049858227641No Hit
AGATGT7650.16470137380322383No Hit
CGGTGG7640.16448607788975558No Hit
ATGTGG7600.16362489423588253No Hit
GGCTTG7510.1616872310146681No Hit
GCAGGT7390.15910368005304892No Hit
GGGGGC7360.15845779231264412No Hit
GGCAGG7340.1580272004857076No Hit
GTGGGT7320.15759660865877106No Hit
GGGGTA7180.15458246587021532No Hit
GGTCTG7160.15415187404327876No Hit
TGCGGA7100.15286009856246918No Hit
GGGGTT7070.15221421082206438No Hit
TGGGTG6860.14769299663923077No Hit
GTTTGC6780.14597062933148466No Hit
TGATGT6740.14510944567761158No Hit
GTTCGG6730.14489414976414333No Hit
GTGGTT6630.14274119062946067No Hit
GAGGTG6630.14274119062946067No Hit
GTGGAG6610.14231059880252414No Hit
ACTTAA6600.14209530288905586No Hit
GGATGG6570.14144941514865106No Hit
TGTCTG6470.1392964560139684No Hit
TACGGG6410.1380046805331588No Hit
TGAGGG6340.13649760913888095No Hit
GGGGTC6310.13585172139847615No Hit
AAGTGG6280.13520583365807134No Hit
AAGTCT6250.13455994591766654No Hit
TTTTTT6230.13412935409072999No Hit
TGTTGG6210.13369876226379346No Hit
GTAGCT6190.13326817043685693No Hit
TGGTTG6060.1304693235617695No Hit
GCAATT6030.12982343582136469No Hit
GTGTTA6000.12917754808095988No Hit
GAGGTA6000.12917754808095988No Hit
GGGCGG5920.12745518077321374No Hit
GGGCAG5920.12745518077321374No Hit
GCTGGG5890.12680929303280894No Hit
GGCGAT5840.1257328134654676No Hit
TGAAAC5830.12551751755199933No Hit
TGGTCG5790.12465633389812628No Hit
GGCTTT5750.12379515024425322No Hit
GGGTTG5710.12293396659038015No Hit
GGCACT5700.12271867067691189No Hit
GGTAGC5700.12271867067691189No Hit
GCGTTG5700.12271867067691189No Hit
GTTTGT5680.12228807884997535No Hit
GTGCAT5650.12164219110957054No Hit
TGTTGT5620.12099630336916574No Hit
AGTAGA5580.12013511971529268No Hit
GTCCTA5550.11948923197488788No Hit
GCTGCC5540.11927393606141962No Hit
AGCTGT5540.11927393606141962No Hit
AATGTG5510.11862804832101481No Hit
TTTGTC5500.11841275240754656No Hit
GGTTTT5490.11819745649407828No Hit
GTATGG5480.11798216058061001No Hit
GTGGCT5470.11776686466714176No Hit
TGCGGG5400.1162597932728639No Hit
TGTGGG5390.11604449735939562No Hit
GGGGGT5370.1156139055324591No Hit
GGACTG5350.11518331370552255No Hit
GCAGGC5340.11496801779205428No Hit
CGAGTT5320.11453742596511775No Hit
TAGTCG5310.1143221300516495No Hit
ACCTTG5290.11389153822471297No Hit
TCATGG5290.11389153822471297No Hit
GGTTAG5280.1136762423112447No Hit
GAGTCT5270.11346094639777642No Hit
AGTCGG5250.11303035457083989No Hit
TCGTGG5210.11216917091696682No Hit
GGCCGC5200.11195387500349856No Hit
TTTGCC5200.11195387500349856No Hit
GCGGGG5190.1117385790900303No Hit
AGGGGG5160.11109269134962549No Hit
GGAGTT5150.11087739543615721No Hit
TAAGTA5150.11087739543615721No Hit
GGTGAG5140.11066209952268896No Hit
GGGGCT5140.11066209952268896No Hit
GTGTAT5130.11044680360922068No Hit
TCTAGG5120.11023150769575243No Hit
GCCTGG5080.10937032404187935No Hit
ATCGGT5070.1091550281284111No Hit
GAAAGT5010.10786325264760148No Hit
ATTGTG5000.10764795673413323No Hit
AGTTTC4980.1072173649071967No Hit
ATCTGG4980.1072173649071967No Hit
GTGAGG4960.10678677308026018No Hit
GTAATG4920.10592558942638709No Hit
TTAGGG4920.10592558942638709No Hit
TCCTGG4900.10549499759945057No Hit
TATTAG4900.10549499759945057No Hit
GTGAAG4900.10549499759945057No Hit
TTTCTT4890.1052797016859823No Hit
GTCGCG4890.1052797016859823No Hit
GGGGTG4870.10484910985904576No Hit
GGATGA4870.10484910985904576No Hit
AAGCTC4870.10484910985904576No Hit
AGGGTC4850.10441851803210922No Hit
GCGCGG4830.10398792620517269No Hit
TCTGAA4820.10377263029170444No Hit
GGCCCC4800.10334203846476789No Hit
GACTAC4800.10334203846476789No Hit
TGGTGG4790.10312674255129964No Hit
GTGGGG4790.10312674255129964No Hit
AGACAA4770.10269615072436311No Hit
GGGTGG4770.10269615072436311No Hit
AGCAGG4750.10226555889742657No Hit
GTGGTG4750.10226555889742657No Hit
GCCTTA4740.1020502629839583No Hit
GGTGTG4730.10183496707049003No Hit
GTCGCA4730.10183496707049003No Hit
TAGGTT4720.10161967115702177No Hit
TGAACG4710.1014043752435535No Hit
GTTAGG4700.10118907933008524No Hit
TTGGTT4700.10118907933008524No Hit
TCAGAG4690.10097378341661696No Hit
GAGTGG4690.10097378341661696No Hit
ATTTAG4690.10097378341661696No Hit
GGTTGG4670.10054319158968043No Hit
TGCCAC4660.10032789567621216No Hit
GGCGTG4650.1001125997627439No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)