FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483840

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483840
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13535789
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT5150733.805267650079356Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT358420.2647943167553809Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT184720.13646784830939668Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT168920.12479508952156391Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT153350.1132922506401363Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT1069050.0127.957992
ACTGCAA1089100.0126.057081
TGCAATT1215050.0122.36423
ACCGCAA141400.0120.317081
CCGCAAT143300.0118.835792
CGCAATT154050.0117.719783
GCAATTT1547000.0109.389314
AATTTCC1708900.0107.6623466
CAATTTC1640650.0106.549145
TTTCCAC1761600.0103.833568
ATTTCCA1780400.0103.042497
TTCCACA1850950.098.962239
GACCGGA44500.072.252752
CGGCAAT42100.071.706432
AGACCGG49400.064.812361
ACGGCAA52900.056.8102261
GTTCCAC122350.056.777385
ACCGGAA61100.052.7413673
GATTGGA137000.049.3803832
AGATTGG137800.048.6870961
Produced by FastQC (version 0.11.5)