FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483842

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483842
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9779883
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT3486423.5648892732152317Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT148200.1515355551799546Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT117570.1202161620951907Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT98760.10098280316850416Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT839750.0129.53532
TGCAATT915250.0126.8305053
ACTGCAA849050.0126.8085861
CGCAATT109950.0120.800663
CCGCAAT106650.0119.1605452
ACCGCAA108500.0116.164691
GCAATTT1150750.0113.950294
AATTTCC1284350.0112.304976
CAATTTC1229350.0111.149025
TTTCCAC1330300.0107.7519158
ATTTCCA1336250.0107.6680157
TTCCACA1394450.0103.159749
CGGCAAT31650.074.340692
GACCGGA36850.067.3975142
AGACCGG38750.061.865231
GTTCCAC85800.059.582155
ACGGCAA39200.058.7459031
ACCGGAA51450.048.4127273
GGCAATT56600.043.751083
GCAAGTT79850.043.3958171
Produced by FastQC (version 0.11.5)