FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005483844

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483844
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences665375
Sequences flagged as poor quality0
Sequence length151
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT17347926.072365207589705Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT45460.6832237460078903Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT39620.5954536915273342Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT28900.4343415367274094Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT27850.41856096186361075Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTC26360.39616757467593466Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGT18830.2829983092241217Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT13320.20018786398647379Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGGTCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT11700.17584069133947022Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAA11310.16997933496148787No Hit
AGATCGGAAAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC10900.16381739620514746RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTCTGCCGTCTT10490.15765545744880705Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTA10190.15314672177343605Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC10030.15074206274657148RNA PCR Primer, Index 16 (96% over 25bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGCAAAA9360.1406725530715762No Hit
AGATCGGACGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT9280.1394702235581439Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAT8050.12098440728912267No Hit
AGATCGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC7590.1140710125868871Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
AGATCGGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCG6730.10114597031749015Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)